Regulation of very primitive, multipotent, hemopoietic cells by hemopoietin-1

E. Richard Stanley, Anna Bartocci, Deborah Patinkin, Martin Rosendaal, T. Ray Bradley

Research output: Contribution to journalArticle

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Abstract

Hemopoietin-1 (H-1) is known to act synergistically with CSF-1, a mononuclear phagocyte growth factor, to induce the development of primitive hemopoietic cells. To determine whether purified H-1 also acts on multipotent hemopoietic cells, its ability to act synergistically with interleukin-3 (IL-3) and erythropoietin (Epo) was tested in methyl cellulose cultures of murine bone marrow cells. In the presence of IL-3, H-1 increased the number of colonies formed by primitive, multipotent cells by ∼30-fold. H-1 alone or H-1 plus Epo produced no colonies. Forty percent of the colonies induced by H-1 plus IL-3 contained cells that could be subcultured at least twice, whereas cells from colonies induced by IL-3 alone could not be similarly subcultured. Thus H-1 permits CSF-1 or IL-3 to act on cells more primitive than those acted on by either growth factor alone. The results indicate that H-1 acts on the most primitive hemopoletic cells yet shown to proliferate and differentiate in culture.

Original languageEnglish (US)
Pages (from-to)667-674
Number of pages8
JournalCell
Volume45
Issue number5
DOIs
StatePublished - Jun 6 1986

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Interleukin-3
Macrophage Colony-Stimulating Factor
Erythropoietin
Intercellular Signaling Peptides and Proteins
Methylcellulose
Cell culture
Bone
Phagocytes
Cells
Bone Marrow Cells

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Stanley, E. R., Bartocci, A., Patinkin, D., Rosendaal, M., & Bradley, T. R. (1986). Regulation of very primitive, multipotent, hemopoietic cells by hemopoietin-1. Cell, 45(5), 667-674. https://doi.org/10.1016/0092-8674(86)90781-6

Regulation of very primitive, multipotent, hemopoietic cells by hemopoietin-1. / Stanley, E. Richard; Bartocci, Anna; Patinkin, Deborah; Rosendaal, Martin; Bradley, T. Ray.

In: Cell, Vol. 45, No. 5, 06.06.1986, p. 667-674.

Research output: Contribution to journalArticle

Stanley, ER, Bartocci, A, Patinkin, D, Rosendaal, M & Bradley, TR 1986, 'Regulation of very primitive, multipotent, hemopoietic cells by hemopoietin-1', Cell, vol. 45, no. 5, pp. 667-674. https://doi.org/10.1016/0092-8674(86)90781-6
Stanley ER, Bartocci A, Patinkin D, Rosendaal M, Bradley TR. Regulation of very primitive, multipotent, hemopoietic cells by hemopoietin-1. Cell. 1986 Jun 6;45(5):667-674. https://doi.org/10.1016/0092-8674(86)90781-6
Stanley, E. Richard ; Bartocci, Anna ; Patinkin, Deborah ; Rosendaal, Martin ; Bradley, T. Ray. / Regulation of very primitive, multipotent, hemopoietic cells by hemopoietin-1. In: Cell. 1986 ; Vol. 45, No. 5. pp. 667-674.
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