Regulation of timing of replication

Rituparna Mukhopadhyay, Eric E. Bouhassira

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Introduction to DNA replication DNA replication is a process fundamental to cell proliferation. Eukaryotic cells must replicate their DNA once and only once per cell cycle. In addition, after each DNA replication, the epigenetic information, such as DNA methylation and histone modifications, that constitute the memory and the identity of the cells must also be replicated. These functional constraints might have caused eukaryotes to evolve complex tissue specific replication programs, perhaps because the timing during S phase at which each DNA segment replicates is critical for the transmission of epigenetic memory. DNA replication begins by the recruitment of the replication machinery at specific sites in the genome called “origins.” DNA replicated from a single origin is referred to as a replicon. DNA synthesis in each replicon is initiated at an origin and proceeds bidirectionally. These replicons eventually fuse resulting in complete genomic duplication. Initiation of DNA replication involves the following steps. Origin recognition The origin recognition complex (ORC) was originally identified in budding yeast and is evolutionally conserved across species (Bell and Stillman, 1992). It is composed of six subunits (ORC1 to ORC6) that act as initiators and bind to the origin sites in eukaryotic DNA. Mammalian ORC consists of a stable core complex of subunits ORC 2, 3, 4, and 5 which interact feebly with ORC1 and ORC6.

Original languageEnglish (US)
Title of host publicationEpigenomics: From Chromatin Biology to Therapeutics
PublisherCambridge University Press
Pages179-194
Number of pages16
ISBN (Print)9780511777271, 9781107003828
DOIs
StatePublished - Jan 1 2012

Fingerprint

Origin Recognition Complex
DNA Replication
Replicon
DNA
Epigenomics
Histone Code
Saccharomycetales
Eukaryotic Cells
DNA Methylation
Eukaryota
S Phase
Cell Cycle
Cell Proliferation
Genome
Data storage equipment
Cell proliferation
Electric fuses
Histones
Yeast
Machinery

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Mukhopadhyay, R., & Bouhassira, E. E. (2012). Regulation of timing of replication. In Epigenomics: From Chromatin Biology to Therapeutics (pp. 179-194). Cambridge University Press. https://doi.org/10.1017/CBO9780511777271.017

Regulation of timing of replication. / Mukhopadhyay, Rituparna; Bouhassira, Eric E.

Epigenomics: From Chromatin Biology to Therapeutics. Cambridge University Press, 2012. p. 179-194.

Research output: Chapter in Book/Report/Conference proceedingChapter

Mukhopadhyay, R & Bouhassira, EE 2012, Regulation of timing of replication. in Epigenomics: From Chromatin Biology to Therapeutics. Cambridge University Press, pp. 179-194. https://doi.org/10.1017/CBO9780511777271.017
Mukhopadhyay R, Bouhassira EE. Regulation of timing of replication. In Epigenomics: From Chromatin Biology to Therapeutics. Cambridge University Press. 2012. p. 179-194 https://doi.org/10.1017/CBO9780511777271.017
Mukhopadhyay, Rituparna ; Bouhassira, Eric E. / Regulation of timing of replication. Epigenomics: From Chromatin Biology to Therapeutics. Cambridge University Press, 2012. pp. 179-194
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