Regulation of the Drosophila ID protein extra macrochaetae by proneural dimerization partners

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Proneural bHLH proteins are transcriptional regulators of neural fate specification. Extra macrochaetae (Emc) forms inactive heterodimers with both proneural bHLH proteins and their bHLH partners (represented in Drosophila by Daughterless). It is generally thought that varying levels of Emc define a prepattern that determines where proneural bHLH genes can be effective. We report that instead it is the bHLH proteins that determine the pattern of Emc levels. Daughterless level sets Emc protein levels in most cells, apparently by stabilizing Emc in heterodimers. Emc is destabilized in proneural regions by local competition for heterodimer formation by proneural bHLH proteins including Atonal or AS-C proteins. Reflecting this post-translational control through protein stability, uniform emc transcription is sufficient for almost normal patterns of neurogenesis. Protein stability regulated by exchanges between bHLH protein dimers could be a feature of bHLH-mediated developmental events.

Original languageEnglish (US)
Article numbere33967
JournaleLife
Volume7
DOIs
StatePublished - Apr 24 2018

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Basic Helix-Loop-Helix Transcription Factors
Drosophila Proteins
Dimerization
Protein Stability
Proteins
Neurogenesis
Transcription
Protein C
Dimers
Drosophila
Genes
Specifications

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Regulation of the Drosophila ID protein extra macrochaetae by proneural dimerization partners. / Li, Ke; Baker, Nicholas E.

In: eLife, Vol. 7, e33967, 24.04.2018.

Research output: Contribution to journalArticle

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