Regulation of synaptic extracellular matrix composition is critical for proper synapse morphology

Peri T. Kurshan, Allan Q. Phan, George J. Wang, Matthew M. Crane, Hang Lu, Kang Shen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Synapses are surrounded by a layer of extracellular matrix (ECM), which is instrumental for their development and maintenance. ECM composition is dynamically controlled by proteases, but how the precise composition of the ECM affects synaptic morphology is largely unknown. Through an unbiased forward genetic screen, we found that Caenorhabditis elegans gon-1, a conserved extracellular ADAMTS protease, is required for maintaining proper synaptic morphology at the neuromuscular junction. In gon-1 mutants, once synapse formation is complete, motor neuron presynaptic varicosities develop into large bulbous protrusions that contain synaptic vesicles and active zone proteins. A concomitant overgrowth of postsynaptic muscle membrane is found in close apposition to presynaptic axonal protrusions. Mutations in the muscle-specific, actin-severing protein cofilin (unc-60) suppress the axon phenotype, suggesting that muscle outgrowth is necessary for presynaptic protrusions. gon-1 mutants can also be suppressed by loss of the ECM components collagen IV (EMB-9) and fibulin (FBL-1). We propose that GON-1 regulates a developmental switch out of an initial “pro-growth” phase during which muscle arms grow out and form synapses with motor neuron axons. We postulate that this switch involves degradation or reorganization of collagen IV (EMB-9), whereas FBL-1 opposes GON-1 by stabilizing EMB-9. Our results describe a mechanism for regulating synaptic ECM composition and reveal the importance of precise ECM composition for neuronal morphology and synapse integrity.

Original languageEnglish (US)
Pages (from-to)12678-12689
Number of pages12
JournalJournal of Neuroscience
Volume34
Issue number38
DOIs
StatePublished - Sep 17 2014
Externally publishedYes

Fingerprint

Synapses
Extracellular Matrix
Muscles
Motor Neurons
Axons
Collagen
Actin Depolymerizing Factors
Synaptic Vesicles
Neuromuscular Junction
Caenorhabditis elegans
Actins
Peptide Hydrolases
Maintenance
Phenotype
Mutation
Membranes
Growth
Proteins

Keywords

  • Extracellular matrix
  • Metalloproteinase
  • Synapse

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Regulation of synaptic extracellular matrix composition is critical for proper synapse morphology. / Kurshan, Peri T.; Phan, Allan Q.; Wang, George J.; Crane, Matthew M.; Lu, Hang; Shen, Kang.

In: Journal of Neuroscience, Vol. 34, No. 38, 17.09.2014, p. 12678-12689.

Research output: Contribution to journalArticle

Kurshan, Peri T. ; Phan, Allan Q. ; Wang, George J. ; Crane, Matthew M. ; Lu, Hang ; Shen, Kang. / Regulation of synaptic extracellular matrix composition is critical for proper synapse morphology. In: Journal of Neuroscience. 2014 ; Vol. 34, No. 38. pp. 12678-12689.
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