Regulation of host cell cyclin D1 by Trypanosoma cruzi in myoblasts

Boumediene Bouzahzah; Vyacheslav Yurchenko; Fnu Nagajyothi; James Hulit; Moshe Sadofsky; Vicki L. Braunstein; Shankar Mukherjee; Hannah Weiss; Fabiana S. Machado; Richard G. Pestell; Michael P. Lisanti; Herbert B. Tanowitz; Chris Albanese

doi:10.4161/cc.7.4.5327

Regulation of host cell cyclin D1 by Trypanosoma cruzi in myoblasts

Boumediene Bouzahzah, Vyacheslav Yurchenko, Fnu Nagajyothi, James Hulit, Moshe Sadofsky, Vicki L. Braunstein, Shankar Mukherjee, Hannah Weiss, Fabiana S. Machado, Richard G. Pestell, Michael P. Lisanti, Herbert B. Tanowitz, Chris Albanese

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Infection with the parasite Trypanosoma cruzi causes Chagas disease. In this study we demonstrated that there was an increase in cyclin D1 expression in T. cruzi (Tulahuen strain)-infected myoblasts. To examine a possible mechanism for the increased cyclin D1 expression we transfected L₆E₉ myoblasts with cyclin D1 luciferase reporter constructs and infected with T. cruzi. There was no evidence of an increase in promoter activity. Additionally, quantitative PCR did not demonstrate any change in cyclin D1 message during infection. Moreover, we demonstrated that the cyclin D1 protein was significantly stabilized after infection. Collectively, these data indicate that infection with T. cruzi increases cyclin D1 protein abundance post-translationally.

Original language	English (US)
Pages (from-to)	500-503
Number of pages	4
Journal	Cell Cycle
Volume	7
Issue number	4
DOIs	https://doi.org/10.4161/cc.7.4.5327
State	Published - Feb 15 2008
Externally published	Yes

Keywords

Cell cycle regulatory proteins
Chagas disease
Cyclin D1
Cyclin D1 promoter-luciferase (CD1Luc) reporters
Trypanosoma cruzi

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4161/cc.7.4.5327

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@article{b46b7c809ab54919bbe1ad4a29137d27,

title = "Regulation of host cell cyclin D1 by Trypanosoma cruzi in myoblasts",

abstract = "Infection with the parasite Trypanosoma cruzi causes Chagas disease. In this study we demonstrated that there was an increase in cyclin D1 expression in T. cruzi (Tulahuen strain)-infected myoblasts. To examine a possible mechanism for the increased cyclin D1 expression we transfected L6E9 myoblasts with cyclin D1 luciferase reporter constructs and infected with T. cruzi. There was no evidence of an increase in promoter activity. Additionally, quantitative PCR did not demonstrate any change in cyclin D1 message during infection. Moreover, we demonstrated that the cyclin D1 protein was significantly stabilized after infection. Collectively, these data indicate that infection with T. cruzi increases cyclin D1 protein abundance post-translationally.",

keywords = "Cell cycle regulatory proteins, Chagas disease, Cyclin D1, Cyclin D1 promoter-luciferase (CD1Luc) reporters, Trypanosoma cruzi",

author = "Boumediene Bouzahzah and Vyacheslav Yurchenko and Fnu Nagajyothi and James Hulit and Moshe Sadofsky and Braunstein, {Vicki L.} and Shankar Mukherjee and Hannah Weiss and Machado, {Fabiana S.} and Pestell, {Richard G.} and Lisanti, {Michael P.} and Tanowitz, {Herbert B.} and Chris Albanese",

note = "Funding Information: This work was supported by NIH grants AI052739 and AI076284 (HBT), CA129003 (CA) and AHA SDG 073525N (SM).",

year = "2008",

month = feb,

day = "15",

doi = "10.4161/cc.7.4.5327",

language = "English (US)",

volume = "7",

pages = "500--503",

journal = "Cell Cycle",

issn = "1538-4101",

publisher = "Landes Bioscience",

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}

TY - JOUR

T1 - Regulation of host cell cyclin D1 by Trypanosoma cruzi in myoblasts

AU - Bouzahzah, Boumediene

AU - Yurchenko, Vyacheslav

AU - Nagajyothi, Fnu

AU - Hulit, James

AU - Sadofsky, Moshe

AU - Braunstein, Vicki L.

AU - Mukherjee, Shankar

AU - Weiss, Hannah

AU - Machado, Fabiana S.

AU - Pestell, Richard G.

AU - Lisanti, Michael P.

AU - Tanowitz, Herbert B.

AU - Albanese, Chris

N1 - Funding Information: This work was supported by NIH grants AI052739 and AI076284 (HBT), CA129003 (CA) and AHA SDG 073525N (SM).

PY - 2008/2/15

Y1 - 2008/2/15

N2 - Infection with the parasite Trypanosoma cruzi causes Chagas disease. In this study we demonstrated that there was an increase in cyclin D1 expression in T. cruzi (Tulahuen strain)-infected myoblasts. To examine a possible mechanism for the increased cyclin D1 expression we transfected L6E9 myoblasts with cyclin D1 luciferase reporter constructs and infected with T. cruzi. There was no evidence of an increase in promoter activity. Additionally, quantitative PCR did not demonstrate any change in cyclin D1 message during infection. Moreover, we demonstrated that the cyclin D1 protein was significantly stabilized after infection. Collectively, these data indicate that infection with T. cruzi increases cyclin D1 protein abundance post-translationally.

AB - Infection with the parasite Trypanosoma cruzi causes Chagas disease. In this study we demonstrated that there was an increase in cyclin D1 expression in T. cruzi (Tulahuen strain)-infected myoblasts. To examine a possible mechanism for the increased cyclin D1 expression we transfected L6E9 myoblasts with cyclin D1 luciferase reporter constructs and infected with T. cruzi. There was no evidence of an increase in promoter activity. Additionally, quantitative PCR did not demonstrate any change in cyclin D1 message during infection. Moreover, we demonstrated that the cyclin D1 protein was significantly stabilized after infection. Collectively, these data indicate that infection with T. cruzi increases cyclin D1 protein abundance post-translationally.

KW - Cell cycle regulatory proteins

KW - Chagas disease

KW - Cyclin D1

KW - Cyclin D1 promoter-luciferase (CD1Luc) reporters

KW - Trypanosoma cruzi

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AN - SCOPUS:40549132540

SN - 1538-4101

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JO - Cell Cycle

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ER -

Regulation of host cell cyclin D1 by Trypanosoma cruzi in myoblasts

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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