Regulation of host cell cyclin D1 by Trypanosoma cruzi in myoblasts

Boumediene Bouzahzah, Vyacheslav Yurchenko, Fnu Nagajyothi, James Hulit, Moshe Sadofsky, Vicki L. Braunstein, Shankar Mukherjee, Hannah Weiss, Fabiana S. Machado, Richard G. Pestell, Michael P. Lisanti, Herbert B. Tanowitz, Chris Albanese

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Infection with the parasite Trypanosoma cruzi causes Chagas disease. In this study we demonstrated that there was an increase in cyclin D1 expression in T. cruzi (Tulahuen strain)-infected myoblasts. To examine a possible mechanism for the increased cyclin D1 expression we transfected L6E9 myoblasts with cyclin D1 luciferase reporter constructs and infected with T. cruzi. There was no evidence of an increase in promoter activity. Additionally, quantitative PCR did not demonstrate any change in cyclin D1 message during infection. Moreover, we demonstrated that the cyclin D1 protein was significantly stabilized after infection. Collectively, these data indicate that infection with T. cruzi increases cyclin D1 protein abundance post-translationally.

Original languageEnglish (US)
Pages (from-to)500-503
Number of pages4
JournalCell Cycle
Volume7
Issue number4
DOIs
StatePublished - Feb 15 2008

Keywords

  • Cell cycle regulatory proteins
  • Chagas disease
  • Cyclin D1
  • Cyclin D1 promoter-luciferase (CD1Luc) reporters
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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    Bouzahzah, B., Yurchenko, V., Nagajyothi, F., Hulit, J., Sadofsky, M., Braunstein, V. L., Mukherjee, S., Weiss, H., Machado, F. S., Pestell, R. G., Lisanti, M. P., Tanowitz, H. B., & Albanese, C. (2008). Regulation of host cell cyclin D1 by Trypanosoma cruzi in myoblasts. Cell Cycle, 7(4), 500-503. https://doi.org/10.4161/cc.7.4.5327