Regulation of hepatic GLUT8 expression in normal and diabetic models

Naira Gorovits, Lingguang Cui, Julia V. Busik, Mollie Ranalletta, Sylvie Hauguel De-Mouzon, Maureen J. Charron

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

GLUT8 is a novel glucose transporter protein that is widely distributed in tissues including liver, a central organ of regulation of glucose homeostasis. The purpose of the current study was to investigate expression and regulation of hepatic GLUT8 mRNA and protein. Therefore, Northern and immunoblot analysis, semiquantitative RT-PCR, and immunofluorescence microscopy were performed using mouse livers at different stages of embryonic and postnatal development and in type 1 (streprozotocin treated) and type 2 (GLUT4 heterozygous) diabetes. GLUT8 mRNA and protein expression in embryonic liver was differentially regulated depending on the prenatal and postnatal developmental stage of the mice. Immunofluorescence microscopy of liver from wild-type mice demonstrated the highest levels of GLUT8 protein in perivenous hepatocytes pointing to its role in regulation of glycolytic flux. In diabetic scenarios, GLUT8 mRNA levels were correlated with circulating insulin; specifically, GLUT8 mRNA decreased in a type 1 diabetes model and increased in a type 2 diabetes model, suggesting a regulatory role for insulin in GLUT8 mRNA expression. While up-regulation of GLUT8 protein occurred in both models of diabetes, only in streptozotocin diabetic livers was GLUT8 zonation altered. These data demonstrate that GLUT8 mRNA and protein are differentially regulated in liver in response to physiologic and pathologic (diabetes) milieu and suggests that GLUT8 is intimately linked to glucose homeostasis.

Original languageEnglish (US)
Pages (from-to)1703-1711
Number of pages9
JournalEndocrinology
Volume144
Issue number5
DOIs
StatePublished - May 1 2003

Fingerprint

Liver
Messenger RNA
Proteins
Fluorescence Microscopy
Homeostasis
Insulin
Glucose
Facilitative Glucose Transport Proteins
Streptozocin
Type 1 Diabetes Mellitus
Type 2 Diabetes Mellitus
Embryonic Development
Hepatocytes
Up-Regulation
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Gorovits, N., Cui, L., Busik, J. V., Ranalletta, M., De-Mouzon, S. H., & Charron, M. J. (2003). Regulation of hepatic GLUT8 expression in normal and diabetic models. Endocrinology, 144(5), 1703-1711. https://doi.org/10.1210/en.2002-220968

Regulation of hepatic GLUT8 expression in normal and diabetic models. / Gorovits, Naira; Cui, Lingguang; Busik, Julia V.; Ranalletta, Mollie; De-Mouzon, Sylvie Hauguel; Charron, Maureen J.

In: Endocrinology, Vol. 144, No. 5, 01.05.2003, p. 1703-1711.

Research output: Contribution to journalArticle

Gorovits, N, Cui, L, Busik, JV, Ranalletta, M, De-Mouzon, SH & Charron, MJ 2003, 'Regulation of hepatic GLUT8 expression in normal and diabetic models', Endocrinology, vol. 144, no. 5, pp. 1703-1711. https://doi.org/10.1210/en.2002-220968
Gorovits N, Cui L, Busik JV, Ranalletta M, De-Mouzon SH, Charron MJ. Regulation of hepatic GLUT8 expression in normal and diabetic models. Endocrinology. 2003 May 1;144(5):1703-1711. https://doi.org/10.1210/en.2002-220968
Gorovits, Naira ; Cui, Lingguang ; Busik, Julia V. ; Ranalletta, Mollie ; De-Mouzon, Sylvie Hauguel ; Charron, Maureen J. / Regulation of hepatic GLUT8 expression in normal and diabetic models. In: Endocrinology. 2003 ; Vol. 144, No. 5. pp. 1703-1711.
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