Regulation of glucose transporters GLUT-4 and GLUT-1 gene transcription in denervated skeletal muscle

Jared P. Jones, Edward B. Tapscott, Ann Louise Olson, Jeffrey E. Pessin, G. Lynis Dohm

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Because GLUT-4 expression is decreased whereas GLUT-1 expression is increased in denervated skeletal muscle, we examined the effects of denervation on GLUT-4 and GLUT-1 gene transcription. The right hindlimb skeletal muscle of male transgenic mice containing sequential truncations (2,400, 1,639, 1,154, and 730 bp) of the human GLUT-4 promoter linked to the chloramphenacol acyl transferase (CAT) gene was denervated, and the contralateral hindlimb was sham operated. RNase protection analysis revealed that after 72 h denervation decreased CAT mRNA and GLUT-4 mRNA levels 64- 85%, respectively (P < 0.05), in the gastrocnemius muscles. In contrast, denervation of the right hindlimb of male rats increased GLUT-1 gene transcription and GLUT-1 mRNA levels by 94 and 213%, respectively (P < 0.05). In conclusion, GLUT-4 transcription is decreased but GLUT-1 transcription is increased in denervated skeletal muscle, suggesting that the effects of denervation on GLUT-4 and GLUT-1 expression are, in part, transcriptionally mediated. Furthermore, these data indicate that a DNA sequence regulated by denervation is located within 730 bp of the 5'-flanking promoter region of the human GLUT-4 gene.

Original languageEnglish (US)
Pages (from-to)1661-1666
Number of pages6
JournalJournal of Applied Physiology
Volume84
Issue number5
StatePublished - May 1998
Externally publishedYes

Fingerprint

Facilitative Glucose Transport Proteins
Denervation
Skeletal Muscle
Hindlimb
Genes
Transferases
Messenger RNA
5' Flanking Region
Ribonucleases
Genetic Promoter Regions
Transgenic Mice

Keywords

  • Gene expression
  • Innervation
  • Insulin resistance

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Regulation of glucose transporters GLUT-4 and GLUT-1 gene transcription in denervated skeletal muscle. / Jones, Jared P.; Tapscott, Edward B.; Olson, Ann Louise; Pessin, Jeffrey E.; Dohm, G. Lynis.

In: Journal of Applied Physiology, Vol. 84, No. 5, 05.1998, p. 1661-1666.

Research output: Contribution to journalArticle

Jones, Jared P. ; Tapscott, Edward B. ; Olson, Ann Louise ; Pessin, Jeffrey E. ; Dohm, G. Lynis. / Regulation of glucose transporters GLUT-4 and GLUT-1 gene transcription in denervated skeletal muscle. In: Journal of Applied Physiology. 1998 ; Vol. 84, No. 5. pp. 1661-1666.
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