Regulation of gene expression in lectin-stimulated or lymphokine-stimulated T lymphocytes: Effects of cyclosporine

C. John Reed, Michael B. Prystowsky, Peter C. Nowell

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The effects of cyclosporine were examined on gene expression induced in T lymphocytes by mitogenic lectins and interleukin 2 (IL-2). Used at concentrations that inhibited proliferation of human peripheral blood lymphocytes by ≈ 9 0%, CsA suppressed, to different extents, the phytohemagglutinin-stimulated expression of various genes, with levels of mRNAs for IL-2 being inhibited by ≈ 1 0 0%, c-myc and N-ras by ≈ 8 0%, and cfos and IL-2 receptors by ≈ 50%. Comparisons of the actions of CsA on gene expression in a cloned murine T cell (L2), stimulated with concanavalin A or IL-2, demonstrated that CsA specifically blocked the accumulation of mRNAs for the c-myc and p53 protooncogenes when induced by Con A, but not when induced by IL-2. Taken together, these findings indicate that several pathways can control the expression of a particulargene, and suggest that CsA interferes with only some of these regulatory pathways of gene expression in T lymphocytes.

Original languageEnglish (US)
Pages (from-to)85S-89S
JournalTransplantation
Volume46
Issue number2
StatePublished - 1988
Externally publishedYes

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Lymphokines
Gene Expression Regulation
Lectins
Cyclosporine
Interleukin-2
T-Lymphocytes
Gene Expression
Messenger RNA
Interleukin-2 Receptors
Phytohemagglutinins
Regulator Genes
Concanavalin A
Lymphocytes

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Regulation of gene expression in lectin-stimulated or lymphokine-stimulated T lymphocytes : Effects of cyclosporine. / Reed, C. John; Prystowsky, Michael B.; Nowell, Peter C.

In: Transplantation, Vol. 46, No. 2, 1988, p. 85S-89S.

Research output: Contribution to journalArticle

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