Regulation of cytoskeleton organization and paxillin dephosphorylation by cAMP

Studies on murine Y1 adrenal cells

J. D. Han, C. S. Rubin

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Cyclic AMP induces corticosteroid production, differential gene transcription, and cell cycle arrest in adrenal cortex-derived Y1 cells. These responses follow a cAMP-controlled transformation in Y1 cell morphology: the conversion of flat epithelial cells into rounded, highly refractile cells with short processes. Little is known about effector proteins and mechanisms that link activated protein kinase A to the alteration in cell shape. We now report that cAMP causes rapid (≤1 min) and selective tyrosine dephosphorylation of paxillin, a focal adhesion protein. Paxillin is maximally dephosphorylated before other physiological effects of cAMP are detected in Y1 cells. Dephosphopaxillin translocates from focal adhesions to the cytoplasm as stress fibers vanish and F-actin accumulates in membrane ruffles and cytoplasmic aggregates. Remnants of focal adhesion complexes dissociate from the cell cortex and coalesce into large structures that contain aggregated F-actin. Pervanadate, an inhibitor of protein- tyrosine phosphatases, abrogates all effects of cAMP. Conversely, genistein- sensitive protein-tyrosine kinase activity is essential for establishing epithelial morphology and reversing effects of cAMP in Y1 cells. Thus, cAMP/protein kinase A (PKA) actions are initially targeted to focal adhesions and cortical actin cytoskeleton; paxillin is an early and unexpected downstream target in a PKA-mediated signaling pathway, and protein-tyrosine phosphatase activity provides an essential link between PKA activation and the control of cell shape.

Original languageEnglish (US)
Pages (from-to)29211-29215
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number46
DOIs
StatePublished - 1996

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Paxillin
Cyclic AMP-Dependent Protein Kinases
Cytoskeleton
Focal Adhesions
Adhesion
Actins
Protein Tyrosine Phosphatases
Cell Shape
Genistein
Transcription
Cyclic AMP
Protein-Tyrosine Kinases
Tyrosine
Stress Fibers
Adrenal Cortex Hormones
Genes
Chemical activation
Adrenal Cortex
Cells
Cell Cycle Checkpoints

ASJC Scopus subject areas

  • Biochemistry

Cite this

Regulation of cytoskeleton organization and paxillin dephosphorylation by cAMP : Studies on murine Y1 adrenal cells. / Han, J. D.; Rubin, C. S.

In: Journal of Biological Chemistry, Vol. 271, No. 46, 1996, p. 29211-29215.

Research output: Contribution to journalArticle

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