Regulation of a human cardiac actin gene introduced into rat L6 myoblasts suggests a defect in their myogenic program.

R. Hickey, A. Skoultchi, P. Gunning, L. Kedes

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The rat myogenic cell line L6E9 induces skeletal but not cardiac alpha-actin mRNA upon fusion to form myotubes. However, when a human cardiac alpha-actin gene was introduced into L6E9 myoblasts, differentiation of the cells led to the accumulation of human gene transcripts in parallel with those derived from the endogenous skeletal alpha-actin gene. This result demonstrates that factors which direct rat myogenesis can regulate a muscle gene from another species and that the L6E9 cells may have a defect in their ability to activate endogenous cardiac actin gene expression.

Original languageEnglish (US)
Pages (from-to)3287-3290
Number of pages4
JournalMolecular and cellular biology
Volume6
Issue number9
DOIs
Publication statusPublished - Sep 1986
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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