Regulated nuclear entry of the C. elegans Pax-6 transcription factor

Yinhua Zhang; Henrique B. Ferreira; David Greenstein; Andrew Chisholm; Scott W. Emmons

doi:10.1016/S0925-4773(98)00171-3

Regulated nuclear entry of the C. elegans Pax-6 transcription factor

Yinhua Zhang, Henrique B. Ferreira, David Greenstein, Andrew Chisholm, Scott W. Emmons

Genetics

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

It is shown that the C. elegans Pax-6 locus encodes two protein isoforms. One contains a Paired DNA binding domain as well as a homeodomain; the other consists only of the carboxy-terminal portion of the locus encoding the homeodomain. These two isoforms are expressed in a variety of postembryonic cell lineages. In one set of lineages, nuclear localization of a homeodomain-only form (MAB-18 isoform) appears to be under temporal and spatial control. Nuclear localization of MAlt-18 is correlated with the genetic requirement for mab-18 and with activation of a reporter gene driven by a mab-18 promoter. Reporter gene expression is dependent on mab-18 gene activity. It is hypothesized that a positive feedback loop is activated by regulated nuclear entry.

Original language	English (US)
Pages (from-to)	179-187
Number of pages	9
Journal	Mechanisms of Development
Volume	78
Issue number	1-2
DOIs	https://doi.org/10.1016/S0925-4773(98)00171-3
State	Published - 1998

Keywords

Caenorhabditis elegans
Homeodomain protein
Pax-6
mab-18
vab-3

ASJC Scopus subject areas

Embryology
Developmental Biology

Access to Document

10.1016/S0925-4773(98)00171-3

Cite this

@article{c239d717ebac4f488f3a116ccaf36b70,

title = "Regulated nuclear entry of the C. elegans Pax-6 transcription factor",

abstract = "It is shown that the C. elegans Pax-6 locus encodes two protein isoforms. One contains a Paired DNA binding domain as well as a homeodomain; the other consists only of the carboxy-terminal portion of the locus encoding the homeodomain. These two isoforms are expressed in a variety of postembryonic cell lineages. In one set of lineages, nuclear localization of a homeodomain-only form (MAB-18 isoform) appears to be under temporal and spatial control. Nuclear localization of MAlt-18 is correlated with the genetic requirement for mab-18 and with activation of a reporter gene driven by a mab-18 promoter. Reporter gene expression is dependent on mab-18 gene activity. It is hypothesized that a positive feedback loop is activated by regulated nuclear entry.",

keywords = "Caenorhabditis elegans, Homeodomain protein, Pax-6, mab-18, vab-3",

author = "Yinhua Zhang and Ferreira, {Henrique B.} and David Greenstein and Andrew Chisholm and Emmons, {Scott W.}",

note = "Funding Information: We thank members of the Emmons laboratory for their comments on the manuscript. This work was supported by grants from the NIH (GM39353) to S.W.E., the American Cancer Society (DB-149) to D.G., and the NIH (GM54657) to A.C. H.B.F. was a recipient of a Brazilian Research Council (CNPq) Postdoctoral Fellowship. D.G. is the recipient of a Junior Faculty Research Award of the American Cancer Society (JFRA-630). A.C. is an Alfred P. Sloan Research Fellow. S.W.E. is the Siegfried Ullmann Professor of Molecular Genetics.",

year = "1998",

doi = "10.1016/S0925-4773(98)00171-3",

language = "English (US)",

volume = "78",

pages = "179--187",

journal = "Mechanisms of Development",

issn = "0925-4773",

publisher = "Elsevier Ireland Ltd",

number = "1-2",

}

TY - JOUR

T1 - Regulated nuclear entry of the C. elegans Pax-6 transcription factor

AU - Zhang, Yinhua

AU - Ferreira, Henrique B.

AU - Greenstein, David

AU - Chisholm, Andrew

AU - Emmons, Scott W.

N1 - Funding Information: We thank members of the Emmons laboratory for their comments on the manuscript. This work was supported by grants from the NIH (GM39353) to S.W.E., the American Cancer Society (DB-149) to D.G., and the NIH (GM54657) to A.C. H.B.F. was a recipient of a Brazilian Research Council (CNPq) Postdoctoral Fellowship. D.G. is the recipient of a Junior Faculty Research Award of the American Cancer Society (JFRA-630). A.C. is an Alfred P. Sloan Research Fellow. S.W.E. is the Siegfried Ullmann Professor of Molecular Genetics.

PY - 1998

Y1 - 1998

N2 - It is shown that the C. elegans Pax-6 locus encodes two protein isoforms. One contains a Paired DNA binding domain as well as a homeodomain; the other consists only of the carboxy-terminal portion of the locus encoding the homeodomain. These two isoforms are expressed in a variety of postembryonic cell lineages. In one set of lineages, nuclear localization of a homeodomain-only form (MAB-18 isoform) appears to be under temporal and spatial control. Nuclear localization of MAlt-18 is correlated with the genetic requirement for mab-18 and with activation of a reporter gene driven by a mab-18 promoter. Reporter gene expression is dependent on mab-18 gene activity. It is hypothesized that a positive feedback loop is activated by regulated nuclear entry.

AB - It is shown that the C. elegans Pax-6 locus encodes two protein isoforms. One contains a Paired DNA binding domain as well as a homeodomain; the other consists only of the carboxy-terminal portion of the locus encoding the homeodomain. These two isoforms are expressed in a variety of postembryonic cell lineages. In one set of lineages, nuclear localization of a homeodomain-only form (MAB-18 isoform) appears to be under temporal and spatial control. Nuclear localization of MAlt-18 is correlated with the genetic requirement for mab-18 and with activation of a reporter gene driven by a mab-18 promoter. Reporter gene expression is dependent on mab-18 gene activity. It is hypothesized that a positive feedback loop is activated by regulated nuclear entry.

KW - Caenorhabditis elegans

KW - Homeodomain protein

KW - Pax-6

KW - mab-18

KW - vab-3

UR - http://www.scopus.com/inward/record.url?scp=0031698274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031698274&partnerID=8YFLogxK

U2 - 10.1016/S0925-4773(98)00171-3

DO - 10.1016/S0925-4773(98)00171-3

M3 - Article

C2 - 9858725

AN - SCOPUS:0031698274

SN - 0925-4773

VL - 78

SP - 179

EP - 187

JO - Mechanisms of Development

JF - Mechanisms of Development

IS - 1-2

ER -

Regulated nuclear entry of the C. elegans Pax-6 transcription factor

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this