Regulated degradation of Chk1 by chaperone-mediated autophagy in response to DNA damage

Caroline Park, Yousin Suh, Ana Maria Cuervo

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Chaperone-mediated autophagy (CMA) is activated in response to cellular stressors to prevent cellular proteotoxicity through selective degradation of altered proteins in lysosomes. Reduced CMA activity contributes to the decrease in proteome quality in disease and ageing. Here, we report that CMA is also upregulated in response to genotoxic insults and that declined CMA functionality leads to reduced cell survival and genomic instability. This role of CMA in genome quality control is exerted through regulated degradation of activated checkpoint kinase 1 (Chk1) by this pathway after the genotoxic insult. Nuclear accumulation of Chk1 in CMA-deficient cells compromises cell cycle progression and prolongs the time that DNA damage persists in these cells. Furthermore, blockage of CMA leads to hyperphosphorylation and destabilization of the MRN (Mre11-Rad50-Nbs1) complex, which participates in early steps of particular DNA repair pathways. We propose that CMA contributes to maintain genome stability by assuring nuclear proteostasis.

Original languageEnglish (US)
Article number6823
JournalNature Communications
Volume6
DOIs
StatePublished - Apr 16 2015

Fingerprint

genome
Autophagy
DNA Damage
Phosphotransferases
deoxyribonucleic acid
Genes
proteome
Cells
degradation
damage
lysosomes
Degradation
DNA
destabilization
Proteome
quality control
cells
progressions
Quality control
Repair

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Regulated degradation of Chk1 by chaperone-mediated autophagy in response to DNA damage. / Park, Caroline; Suh, Yousin; Cuervo, Ana Maria.

In: Nature Communications, Vol. 6, 6823, 16.04.2015.

Research output: Contribution to journalArticle

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