Regional heterogeneity in expression of the sphingosine-1-phosphate pathway in the female rat lower urinary tract

Katherine S. Sandhu, Rowena G. Chua, Xinhua Zhang, Nirmala Devi Kanika, Sarah A. Collins, Magdy Mikhail, Arnold Melman, Michael E. DiSanto

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Objective: We investigated the existence and regional distribution of sphingosine-1-phosphate regulatory enzymes and receptors in the lower urinary tract and determined the functional role of sphingosine-1-phosphate receptors in the bladder. Study Design: Lower urinary tract tissue from 10 female rats was harvested for real-time reverse transcriptase-polymerase chain reaction or organ bath physiology, whereas blood serum was obtained for high-performance liquid chromatography determination of sphingosine-1-phosphate levels. Statistical analysis included the Student t test and analysis of variance. Results: All 3 sphingosine-1-phosphate receptors and major enzymes were expressed throughout the lower urinary tract, but expression and physiologic force generation varied among regions. Sphingosine-1-phosphate was detected in serum. Conclusion: We provide novel data that the sphingosine-1-phosphate signaling pathway regulatory proteins exist throughout the female rat lower urinary tract, but that relative expression exhibits regional heterogeneity corresponding with lower urinary tract contractile response to sphingosine-1-phosphate. Our study suggests that sphingosine-1-phosphate signaling is important in the lower urinary tract and identifies this pathway as a possible target for altering bladder smooth muscle tone.

Original languageEnglish (US)
Pages (from-to)576.e1-576.e7
JournalAmerican Journal of Obstetrics and Gynecology
Volume200
Issue number5
DOIs
StatePublished - May 2009

Keywords

  • G-protein-coupled receptor
  • overactive bladder
  • rho-kinase
  • smooth muscle
  • sphingosine-1-phosphate

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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    Sandhu, K. S., Chua, R. G., Zhang, X., Kanika, N. D., Collins, S. A., Mikhail, M., Melman, A., & DiSanto, M. E. (2009). Regional heterogeneity in expression of the sphingosine-1-phosphate pathway in the female rat lower urinary tract. American Journal of Obstetrics and Gynecology, 200(5), 576.e1-576.e7. https://doi.org/10.1016/j.ajog.2008.12.007