Regional assignment of the structural gene for human acid β-glucosidase to q42→qter on chromosome 1

E. A. Devine, M. Smith, F. X. Arredondo-Vega, B. Shafit-Zagardo, R. J. Desnick

Research output: Contribution to journalArticle

11 Scopus citations


The structural gene for human acid β-glucosidase (GBA) has been regionally assigned to a narrow region on chromosome 1 using somatic cell hybridization, specific immunoprecipitation, and assay with the natural substrate. A human fibroblast line, 46, XX, del(1)(pter→q42:), was fused with mouse RAG fibroblasts and the heterokaryons were subcloned. All hybrid subclones containing a normal chromosome 1 were positive for GBA. In contrast, subclones with a single del(1) were negative for GBA by both immunoprecipitation and natural substrate assays. These results were consistent with the previous assignment of GBA to the region 1p11→qter and further localized the gene to the narrow region 1q42→qter.

Original languageEnglish (US)
Pages (from-to)340-344
Number of pages5
JournalCytogenetic and Genome Research
Issue number4
Publication statusPublished - Jan 1 1982
Externally publishedYes


ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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