Abstract
The structural gene for human acid β-glucosidase (GBA) has been regionally assigned to a narrow region on chromosome 1 using somatic cell hybridization, specific immunoprecipitation, and assay with the natural substrate. A human fibroblast line, 46, XX, del(1)(pter→q42:), was fused with mouse RAG fibroblasts and the heterokaryons were subcloned. All hybrid subclones containing a normal chromosome 1 were positive for GBA. In contrast, subclones with a single del(1) were negative for GBA by both immunoprecipitation and natural substrate assays. These results were consistent with the previous assignment of GBA to the region 1p11→qter and further localized the gene to the narrow region 1q42→qter.
Original language | English (US) |
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Pages (from-to) | 340-344 |
Number of pages | 5 |
Journal | Cytogenetics and Cell Genetics |
Volume | 33 |
Issue number | 4 |
DOIs | |
State | Published - 1982 |
Externally published | Yes |
ASJC Scopus subject areas
- Genetics
- Cell Biology