Reductive metabolism of ascorbic acid in the central nervous system

Dennis D. Pietronigro; Movses Hovsepian; Harry B. Demopoulos; Eugene S. Flamm

doi:10.1016/0006-8993(85)90138-6

Reductive metabolism of ascorbic acid in the central nervous system

Dennis D. Pietronigro, Movses Hovsepian, Harry B. Demopoulos, Eugene S. Flamm

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Rat and feline brain and feline spinal cord were examined for the presence of semidehydroascorbate reductase (EC 1.6.5.4) and dehydroascorbate reductase (EC 1.8.5.1). Semidehydroascorbate reductase (SDAR), as monitored by both ascorbyl radical-dependent nicotinamide adenine dinucleotide (NADH) oxidase activity and NADH-dependent ascorbyl radical quenching, was present in all tissues studied. Rat cerebrum exhibited the highest levels and feline spinal cord the lowest. SDAR activity was about twice as high in feline cerebral cortex as in underlying white matter, and paralleled ascorbic acid levels. Subcellular fractionation of rat cerebrum localized most SDAR in a large granular fraction. In contrast, dehydroascorbate reductase was not detectable in any of the tissues examined. The results suggest that semidehydroascorbate reductase is the major enzyme catalyzing the regeneration of reduced ascorbic acid in the central nervous system.

Original language	English (US)
Pages (from-to)	161-164
Number of pages	4
Journal	Brain research
Volume	333
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(85)90138-6
State	Published - Apr 29 1985
Externally published	Yes

Keywords

ascorbic acid
central nervous system
metabolism

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(85)90138-6

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title = "Reductive metabolism of ascorbic acid in the central nervous system",

abstract = "Rat and feline brain and feline spinal cord were examined for the presence of semidehydroascorbate reductase (EC 1.6.5.4) and dehydroascorbate reductase (EC 1.8.5.1). Semidehydroascorbate reductase (SDAR), as monitored by both ascorbyl radical-dependent nicotinamide adenine dinucleotide (NADH) oxidase activity and NADH-dependent ascorbyl radical quenching, was present in all tissues studied. Rat cerebrum exhibited the highest levels and feline spinal cord the lowest. SDAR activity was about twice as high in feline cerebral cortex as in underlying white matter, and paralleled ascorbic acid levels. Subcellular fractionation of rat cerebrum localized most SDAR in a large granular fraction. In contrast, dehydroascorbate reductase was not detectable in any of the tissues examined. The results suggest that semidehydroascorbate reductase is the major enzyme catalyzing the regeneration of reduced ascorbic acid in the central nervous system.",

keywords = "ascorbic acid, central nervous system, metabolism",

author = "Pietronigro, {Dennis D.} and Movses Hovsepian and Demopoulos, {Harry B.} and Flamm, {Eugene S.}",

year = "1985",

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doi = "10.1016/0006-8993(85)90138-6",

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T1 - Reductive metabolism of ascorbic acid in the central nervous system

AU - Pietronigro, Dennis D.

AU - Hovsepian, Movses

AU - Demopoulos, Harry B.

AU - Flamm, Eugene S.

PY - 1985/4/29

Y1 - 1985/4/29

N2 - Rat and feline brain and feline spinal cord were examined for the presence of semidehydroascorbate reductase (EC 1.6.5.4) and dehydroascorbate reductase (EC 1.8.5.1). Semidehydroascorbate reductase (SDAR), as monitored by both ascorbyl radical-dependent nicotinamide adenine dinucleotide (NADH) oxidase activity and NADH-dependent ascorbyl radical quenching, was present in all tissues studied. Rat cerebrum exhibited the highest levels and feline spinal cord the lowest. SDAR activity was about twice as high in feline cerebral cortex as in underlying white matter, and paralleled ascorbic acid levels. Subcellular fractionation of rat cerebrum localized most SDAR in a large granular fraction. In contrast, dehydroascorbate reductase was not detectable in any of the tissues examined. The results suggest that semidehydroascorbate reductase is the major enzyme catalyzing the regeneration of reduced ascorbic acid in the central nervous system.

AB - Rat and feline brain and feline spinal cord were examined for the presence of semidehydroascorbate reductase (EC 1.6.5.4) and dehydroascorbate reductase (EC 1.8.5.1). Semidehydroascorbate reductase (SDAR), as monitored by both ascorbyl radical-dependent nicotinamide adenine dinucleotide (NADH) oxidase activity and NADH-dependent ascorbyl radical quenching, was present in all tissues studied. Rat cerebrum exhibited the highest levels and feline spinal cord the lowest. SDAR activity was about twice as high in feline cerebral cortex as in underlying white matter, and paralleled ascorbic acid levels. Subcellular fractionation of rat cerebrum localized most SDAR in a large granular fraction. In contrast, dehydroascorbate reductase was not detectable in any of the tissues examined. The results suggest that semidehydroascorbate reductase is the major enzyme catalyzing the regeneration of reduced ascorbic acid in the central nervous system.

KW - ascorbic acid

KW - central nervous system

KW - metabolism

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JO - Brain Research

JF - Brain Research

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ER -

Reductive metabolism of ascorbic acid in the central nervous system

Abstract

Keywords

ASJC Scopus subject areas

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