Reduction of phosphodiesterase 3B gene expression in peroxisome proliferator-activated receptor γ (+/-) mice independent of adipocyte size

Takahiro Ogura, Haruhiko Osawa, Yan Tang, Hiroshi Onuma, Masaaki Ochi, Tatsuya Nishimiya, Naoto Kubota, Yasuo Terauchi, Takashi Kadowaki, Hideichi Makino

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Phosphodiesterase 3B (PDE3B) gene expression is generally reduced in large adipocytes of obese, insulin-resistant mice. This reduced gene expression is restored by peroxisome proliferator-activated receptor (PPAR) γ ligands accompanied by a reduced fat cell size. To determine whether PDE3B gene expression is regulated by PPARγ itself, we analyzed lean PPARγ (+/-) mice with adipocyte size comparable to control PPARγ (+/+) mice. In adipocytes of PPARγ (+/-) mice, PDE3B mRNA and protein were both reduced to 63% of wild-type levels. Basal PDE activity tended to be decreased to 70% of wild-type levels, and, similarly, insulin-induced PDE activity was significantly decreased to 70%. Thus, PPARγ is required for PDE3B gene expression independent of adipocyte size.

Original languageEnglish (US)
Pages (from-to)65-68
Number of pages4
JournalFEBS Letters
Volume542
Issue number1-3
DOIs
StatePublished - May 8 2003
Externally publishedYes

Fingerprint

Type 3 Cyclic Nucleotide Phosphodiesterases
Peroxisome Proliferator-Activated Receptors
Adipocytes
Gene expression
Gene Expression
Insulin
Cell Size
Fats
Ligands
Messenger RNA

Keywords

  • Adipocyte
  • Insulin
  • Insulin resistance
  • Peroxisome proliferator-activated receptor γ
  • Phosphodiesterase 3B
  • Type 2 diabetes

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Reduction of phosphodiesterase 3B gene expression in peroxisome proliferator-activated receptor γ (+/-) mice independent of adipocyte size. / Ogura, Takahiro; Osawa, Haruhiko; Tang, Yan; Onuma, Hiroshi; Ochi, Masaaki; Nishimiya, Tatsuya; Kubota, Naoto; Terauchi, Yasuo; Kadowaki, Takashi; Makino, Hideichi.

In: FEBS Letters, Vol. 542, No. 1-3, 08.05.2003, p. 65-68.

Research output: Contribution to journalArticle

Ogura, T, Osawa, H, Tang, Y, Onuma, H, Ochi, M, Nishimiya, T, Kubota, N, Terauchi, Y, Kadowaki, T & Makino, H 2003, 'Reduction of phosphodiesterase 3B gene expression in peroxisome proliferator-activated receptor γ (+/-) mice independent of adipocyte size', FEBS Letters, vol. 542, no. 1-3, pp. 65-68. https://doi.org/10.1016/S0014-5793(03)00339-9
Ogura, Takahiro ; Osawa, Haruhiko ; Tang, Yan ; Onuma, Hiroshi ; Ochi, Masaaki ; Nishimiya, Tatsuya ; Kubota, Naoto ; Terauchi, Yasuo ; Kadowaki, Takashi ; Makino, Hideichi. / Reduction of phosphodiesterase 3B gene expression in peroxisome proliferator-activated receptor γ (+/-) mice independent of adipocyte size. In: FEBS Letters. 2003 ; Vol. 542, No. 1-3. pp. 65-68.
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