Reduction of phosphodiesterase 3B gene expression in peroxisome proliferator-activated receptor γ (+/-) mice independent of adipocyte size

Takahiro Ogura; Haruhiko Osawa; Yan Tang; Hiroshi Onuma; Masaaki Ochi; Tatsuya Nishimiya; Naoto Kubota; Yasuo Terauchi; Takashi Kadowaki; Hideichi Makino

doi:10.1016/S0014-5793(03)00339-9

Reduction of phosphodiesterase 3B gene expression in peroxisome proliferator-activated receptor γ (+/-) mice independent of adipocyte size

Takahiro Ogura, Haruhiko Osawa, Yan Tang, Hiroshi Onuma, Masaaki Ochi, Tatsuya Nishimiya, Naoto Kubota, Yasuo Terauchi, Takashi Kadowaki, Hideichi Makino

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Phosphodiesterase 3B (PDE3B) gene expression is generally reduced in large adipocytes of obese, insulin-resistant mice. This reduced gene expression is restored by peroxisome proliferator-activated receptor (PPAR) γ ligands accompanied by a reduced fat cell size. To determine whether PDE3B gene expression is regulated by PPARγ itself, we analyzed lean PPARγ (+/-) mice with adipocyte size comparable to control PPARγ (+/+) mice. In adipocytes of PPARγ (+/-) mice, PDE3B mRNA and protein were both reduced to 63% of wild-type levels. Basal PDE activity tended to be decreased to 70% of wild-type levels, and, similarly, insulin-induced PDE activity was significantly decreased to 70%. Thus, PPARγ is required for PDE3B gene expression independent of adipocyte size.

Original language	English (US)
Pages (from-to)	65-68
Number of pages	4
Journal	FEBS Letters
Volume	542
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(03)00339-9
State	Published - May 8 2003
Externally published	Yes

Keywords

Adipocyte
Insulin
Insulin resistance
Peroxisome proliferator-activated receptor γ
Phosphodiesterase 3B
Type 2 diabetes

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0014-5793(03)00339-9

Cite this

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title = "Reduction of phosphodiesterase 3B gene expression in peroxisome proliferator-activated receptor γ (+/-) mice independent of adipocyte size",

abstract = "Phosphodiesterase 3B (PDE3B) gene expression is generally reduced in large adipocytes of obese, insulin-resistant mice. This reduced gene expression is restored by peroxisome proliferator-activated receptor (PPAR) γ ligands accompanied by a reduced fat cell size. To determine whether PDE3B gene expression is regulated by PPARγ itself, we analyzed lean PPARγ (+/-) mice with adipocyte size comparable to control PPARγ (+/+) mice. In adipocytes of PPARγ (+/-) mice, PDE3B mRNA and protein were both reduced to 63% of wild-type levels. Basal PDE activity tended to be decreased to 70% of wild-type levels, and, similarly, insulin-induced PDE activity was significantly decreased to 70%. Thus, PPARγ is required for PDE3B gene expression independent of adipocyte size.",

keywords = "Adipocyte, Insulin, Insulin resistance, Peroxisome proliferator-activated receptor γ, Phosphodiesterase 3B, Type 2 diabetes",

author = "Takahiro Ogura and Haruhiko Osawa and Yan Tang and Hiroshi Onuma and Masaaki Ochi and Tatsuya Nishimiya and Naoto Kubota and Yasuo Terauchi and Takashi Kadowaki and Hideichi Makino",

note = "Funding Information: This work was supported mainly by a Grant-in-Aid for Scientific Research on Priority Areas (C) {\textquoteleft}Medical Genome Science{\textquoteright} (No. 12204007), Grants-in-Aid for Scientific Research (C) (Nos. 14571097, 14571095, and 14571096) from the Ministry of Education, Culture, Science, Sports and Technology of Japan, and a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan by the Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists (No. 60206-00). We thank F. Tanabe and M. Murase for technical assistance and suggestions.",

year = "2003",

month = may,

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doi = "10.1016/S0014-5793(03)00339-9",

language = "English (US)",

volume = "542",

pages = "65--68",

journal = "FEBS Letters",

issn = "0014-5793",

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number = "1-3",

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TY - JOUR

T1 - Reduction of phosphodiesterase 3B gene expression in peroxisome proliferator-activated receptor γ (+/-) mice independent of adipocyte size

AU - Ogura, Takahiro

AU - Osawa, Haruhiko

AU - Tang, Yan

AU - Onuma, Hiroshi

AU - Ochi, Masaaki

AU - Nishimiya, Tatsuya

AU - Kubota, Naoto

AU - Terauchi, Yasuo

AU - Kadowaki, Takashi

AU - Makino, Hideichi

N1 - Funding Information: This work was supported mainly by a Grant-in-Aid for Scientific Research on Priority Areas (C) ‘Medical Genome Science’ (No. 12204007), Grants-in-Aid for Scientific Research (C) (Nos. 14571097, 14571095, and 14571096) from the Ministry of Education, Culture, Science, Sports and Technology of Japan, and a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan by the Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists (No. 60206-00). We thank F. Tanabe and M. Murase for technical assistance and suggestions.

PY - 2003/5/8

Y1 - 2003/5/8

N2 - Phosphodiesterase 3B (PDE3B) gene expression is generally reduced in large adipocytes of obese, insulin-resistant mice. This reduced gene expression is restored by peroxisome proliferator-activated receptor (PPAR) γ ligands accompanied by a reduced fat cell size. To determine whether PDE3B gene expression is regulated by PPARγ itself, we analyzed lean PPARγ (+/-) mice with adipocyte size comparable to control PPARγ (+/+) mice. In adipocytes of PPARγ (+/-) mice, PDE3B mRNA and protein were both reduced to 63% of wild-type levels. Basal PDE activity tended to be decreased to 70% of wild-type levels, and, similarly, insulin-induced PDE activity was significantly decreased to 70%. Thus, PPARγ is required for PDE3B gene expression independent of adipocyte size.

AB - Phosphodiesterase 3B (PDE3B) gene expression is generally reduced in large adipocytes of obese, insulin-resistant mice. This reduced gene expression is restored by peroxisome proliferator-activated receptor (PPAR) γ ligands accompanied by a reduced fat cell size. To determine whether PDE3B gene expression is regulated by PPARγ itself, we analyzed lean PPARγ (+/-) mice with adipocyte size comparable to control PPARγ (+/+) mice. In adipocytes of PPARγ (+/-) mice, PDE3B mRNA and protein were both reduced to 63% of wild-type levels. Basal PDE activity tended to be decreased to 70% of wild-type levels, and, similarly, insulin-induced PDE activity was significantly decreased to 70%. Thus, PPARγ is required for PDE3B gene expression independent of adipocyte size.

KW - Adipocyte

KW - Insulin

KW - Insulin resistance

KW - Peroxisome proliferator-activated receptor γ

KW - Phosphodiesterase 3B

KW - Type 2 diabetes

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ER -

Reduction of phosphodiesterase 3B gene expression in peroxisome proliferator-activated receptor γ (+/-) mice independent of adipocyte size

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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