Reduction of phosphodiesterase 3B gene expression in peroxisome proliferator-activated receptor γ (+/-) mice independent of adipocyte size

Takahiro Ogura, Haruhiko Osawa, Yan Tang, Hiroshi Onuma, Masaaki Ochi, Tatsuya Nishimiya, Naoto Kubota, Yasuo Terauchi, Takashi Kadowaki, Hideichi Makino

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Phosphodiesterase 3B (PDE3B) gene expression is generally reduced in large adipocytes of obese, insulin-resistant mice. This reduced gene expression is restored by peroxisome proliferator-activated receptor (PPAR) γ ligands accompanied by a reduced fat cell size. To determine whether PDE3B gene expression is regulated by PPARγ itself, we analyzed lean PPARγ (+/-) mice with adipocyte size comparable to control PPARγ (+/+) mice. In adipocytes of PPARγ (+/-) mice, PDE3B mRNA and protein were both reduced to 63% of wild-type levels. Basal PDE activity tended to be decreased to 70% of wild-type levels, and, similarly, insulin-induced PDE activity was significantly decreased to 70%. Thus, PPARγ is required for PDE3B gene expression independent of adipocyte size.

Original languageEnglish (US)
Pages (from-to)65-68
Number of pages4
JournalFEBS Letters
Volume542
Issue number1-3
DOIs
StatePublished - May 8 2003
Externally publishedYes

Keywords

  • Adipocyte
  • Insulin
  • Insulin resistance
  • Peroxisome proliferator-activated receptor γ
  • Phosphodiesterase 3B
  • Type 2 diabetes

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Ogura, T., Osawa, H., Tang, Y., Onuma, H., Ochi, M., Nishimiya, T., Kubota, N., Terauchi, Y., Kadowaki, T., & Makino, H. (2003). Reduction of phosphodiesterase 3B gene expression in peroxisome proliferator-activated receptor γ (+/-) mice independent of adipocyte size. FEBS Letters, 542(1-3), 65-68. https://doi.org/10.1016/S0014-5793(03)00339-9