Reduction and renaturation of hen egg lysozyme containing carboxymethylcysteine-6 and -127

A. Seetharama Acharya

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The thermodynamic stability of an enzymatically active derivative (sample LHO of hen egg lysozyme containing three, presumably, native disulfide bonds and carboxymethylated cysteines at positions 6 and 127 and exhibiting characteristics of the intact enzyme has been studied with respect to the formation of disulfide bonds. The procedure is to measure the equilibrium between the native and nonnative forms, after reequilibrating the disulfide bonds in the presence of β-mercaptoethanol, by gel filtration on Bio-Gel P-30. The native form has a lower hydrodynamic volume than the nonnative forms. To test whether the equilibrium has been reached, three different disulfide bonded forms of sample LH1 (native, reduced, and "scrambled") are exposed to the same concen-tration of β-mercaptoethanol (1.5 mM) at pH 8.0 and 37°C, which permits the disulfide interchange. All three samples show similar ratios (3:2) of native to nonnative forms after 16 h. This ratio is reached within 5 h in the case of native sample LH1. Consequently the apparent equilibrium appears to be reached. In these same conditions intact lysozyme shows only the native form. Thus, sample LH1 is less stable than the intact protein with respect to disulfide bond formation, but more stable than the nonnative isomers of sample LH1 obtained by reshuffling the disulfide bonds. This latter point has been tested by measuring the proportions of all the partially reduced forms of sample LH1 by trapping the free sulfhydryl groups present in the equilibrium mixture with iodoacetic acid.

Original languageEnglish (US)
Pages (from-to)3064-3070
Number of pages7
JournalBiochemistry
Volume17
Issue number15
StatePublished - 1978
Externally publishedYes

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Disulfides
Mercaptoethanol
Gels
Iodoacetic Acid
Interchanges
Hydrodynamics
Muramidase
hen egg lysozyme
Thermodynamics
Isomers
Gel Chromatography
Cysteine
Thermodynamic stability
Derivatives
Enzymes
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Reduction and renaturation of hen egg lysozyme containing carboxymethylcysteine-6 and -127. / Seetharama Acharya, A.

In: Biochemistry, Vol. 17, No. 15, 1978, p. 3064-3070.

Research output: Contribution to journalArticle

Seetharama Acharya, A. / Reduction and renaturation of hen egg lysozyme containing carboxymethylcysteine-6 and -127. In: Biochemistry. 1978 ; Vol. 17, No. 15. pp. 3064-3070.
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