Reduced neurotrophin receptor tropomyosin-related kinase A expression in human granulosa cells

A novel marker of diminishing ovarian reserve

Erkan Buyuk, Nanette Santoro, Hillel W. Cohen, Maureen J. Charron, Sangita K. Jindal

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: To determine whether human mural and cumulus granulosa cell neurotrophin and neurotrophin receptor content correlate to ovarian reserve markers. Design: Prospective, laboratory-based study. Setting: Academic assisted reproductive technology (ART) program. Patient(s): Twenty-three women undergoing ART. Intervention(s): Mural and cumulus granulosa cells were collected from women undergoing oocyte retrieval during ART cycles. Relative messenger RNA (mRNA) levels of neurotrophins and their receptors were measured by quantitative reverse transcription-polymerase chain reaction and correlated to serum antimüllerian hormone (AMH) levels and the number of oocytes retrieved. Main Outcome Measure(s): Number of oocytes retrieved. Result(s): Mural and cumulus granulosa cell nerve growth factor receptor tropomyosin-related kinase A (TrkA) mRNA correlated strongly to the number of oocytes retrieved. Similarly, higher serum AMH was associated with higher cumulus granulosa cell TrkA mRNA. Both mural and cumulus granulosa cell p75 NTR/TrkA ratios were lower in women with higher serum AMH, and the number of oocytes retrieved was greater among women with low p75 NTR/TrkA ratio. No significant associations were found between brain-derived neurotrophic factor (BDNF) and its specific receptor tropomyosin-related kinase B (TrkB) and ovarian reserve markers. Although BDNF and TrkB expression were higher in cumulus compared with mural granulosa cells, no such association was found between TrkA and granulosa cells. Antimüllerian hormone and cumulus TrkA mRNA, in a model incorporating both, correlated strongly to the number of oocytes retrieved (R2 = 0.84). Conclusion(s): Cumulus TrkA and p75NTR mRNA correlate to ovarian reserve, whereas BDNF and TrkB are associated with the type of granulosa cell.

Original languageEnglish (US)
JournalFertility and Sterility
Volume96
Issue number2
DOIs
StatePublished - Aug 2011

Fingerprint

Nerve Growth Factor Receptors
Granulosa Cells
Cumulus Cells
Oocytes
Assisted Reproductive Techniques
Brain-Derived Neurotrophic Factor
Messenger RNA
Hormones
Serum
tropomyosin kinase
Ovarian Reserve
Oocyte Retrieval
Nerve Growth Factor Receptor
Nerve Growth Factors
Reverse Transcription
Outcome Assessment (Health Care)

Keywords

  • antimüllerian hormone
  • cumulus granulosa cell
  • diminished ovarian reserve
  • mural
  • TrkA

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

@article{961f5bd54e1b40f09dc43f70eb006e40,
title = "Reduced neurotrophin receptor tropomyosin-related kinase A expression in human granulosa cells: A novel marker of diminishing ovarian reserve",
abstract = "Objective: To determine whether human mural and cumulus granulosa cell neurotrophin and neurotrophin receptor content correlate to ovarian reserve markers. Design: Prospective, laboratory-based study. Setting: Academic assisted reproductive technology (ART) program. Patient(s): Twenty-three women undergoing ART. Intervention(s): Mural and cumulus granulosa cells were collected from women undergoing oocyte retrieval during ART cycles. Relative messenger RNA (mRNA) levels of neurotrophins and their receptors were measured by quantitative reverse transcription-polymerase chain reaction and correlated to serum antim{\"u}llerian hormone (AMH) levels and the number of oocytes retrieved. Main Outcome Measure(s): Number of oocytes retrieved. Result(s): Mural and cumulus granulosa cell nerve growth factor receptor tropomyosin-related kinase A (TrkA) mRNA correlated strongly to the number of oocytes retrieved. Similarly, higher serum AMH was associated with higher cumulus granulosa cell TrkA mRNA. Both mural and cumulus granulosa cell p75 NTR/TrkA ratios were lower in women with higher serum AMH, and the number of oocytes retrieved was greater among women with low p75 NTR/TrkA ratio. No significant associations were found between brain-derived neurotrophic factor (BDNF) and its specific receptor tropomyosin-related kinase B (TrkB) and ovarian reserve markers. Although BDNF and TrkB expression were higher in cumulus compared with mural granulosa cells, no such association was found between TrkA and granulosa cells. Antim{\"u}llerian hormone and cumulus TrkA mRNA, in a model incorporating both, correlated strongly to the number of oocytes retrieved (R2 = 0.84). Conclusion(s): Cumulus TrkA and p75NTR mRNA correlate to ovarian reserve, whereas BDNF and TrkB are associated with the type of granulosa cell.",
keywords = "antim{\"u}llerian hormone, cumulus granulosa cell, diminished ovarian reserve, mural, TrkA",
author = "Erkan Buyuk and Nanette Santoro and Cohen, {Hillel W.} and Charron, {Maureen J.} and Jindal, {Sangita K.}",
year = "2011",
month = "8",
doi = "10.1016/j.fertnstert.2011.05.017",
language = "English (US)",
volume = "96",
journal = "Fertility and Sterility",
issn = "0015-0282",
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TY - JOUR

T1 - Reduced neurotrophin receptor tropomyosin-related kinase A expression in human granulosa cells

T2 - A novel marker of diminishing ovarian reserve

AU - Buyuk, Erkan

AU - Santoro, Nanette

AU - Cohen, Hillel W.

AU - Charron, Maureen J.

AU - Jindal, Sangita K.

PY - 2011/8

Y1 - 2011/8

N2 - Objective: To determine whether human mural and cumulus granulosa cell neurotrophin and neurotrophin receptor content correlate to ovarian reserve markers. Design: Prospective, laboratory-based study. Setting: Academic assisted reproductive technology (ART) program. Patient(s): Twenty-three women undergoing ART. Intervention(s): Mural and cumulus granulosa cells were collected from women undergoing oocyte retrieval during ART cycles. Relative messenger RNA (mRNA) levels of neurotrophins and their receptors were measured by quantitative reverse transcription-polymerase chain reaction and correlated to serum antimüllerian hormone (AMH) levels and the number of oocytes retrieved. Main Outcome Measure(s): Number of oocytes retrieved. Result(s): Mural and cumulus granulosa cell nerve growth factor receptor tropomyosin-related kinase A (TrkA) mRNA correlated strongly to the number of oocytes retrieved. Similarly, higher serum AMH was associated with higher cumulus granulosa cell TrkA mRNA. Both mural and cumulus granulosa cell p75 NTR/TrkA ratios were lower in women with higher serum AMH, and the number of oocytes retrieved was greater among women with low p75 NTR/TrkA ratio. No significant associations were found between brain-derived neurotrophic factor (BDNF) and its specific receptor tropomyosin-related kinase B (TrkB) and ovarian reserve markers. Although BDNF and TrkB expression were higher in cumulus compared with mural granulosa cells, no such association was found between TrkA and granulosa cells. Antimüllerian hormone and cumulus TrkA mRNA, in a model incorporating both, correlated strongly to the number of oocytes retrieved (R2 = 0.84). Conclusion(s): Cumulus TrkA and p75NTR mRNA correlate to ovarian reserve, whereas BDNF and TrkB are associated with the type of granulosa cell.

AB - Objective: To determine whether human mural and cumulus granulosa cell neurotrophin and neurotrophin receptor content correlate to ovarian reserve markers. Design: Prospective, laboratory-based study. Setting: Academic assisted reproductive technology (ART) program. Patient(s): Twenty-three women undergoing ART. Intervention(s): Mural and cumulus granulosa cells were collected from women undergoing oocyte retrieval during ART cycles. Relative messenger RNA (mRNA) levels of neurotrophins and their receptors were measured by quantitative reverse transcription-polymerase chain reaction and correlated to serum antimüllerian hormone (AMH) levels and the number of oocytes retrieved. Main Outcome Measure(s): Number of oocytes retrieved. Result(s): Mural and cumulus granulosa cell nerve growth factor receptor tropomyosin-related kinase A (TrkA) mRNA correlated strongly to the number of oocytes retrieved. Similarly, higher serum AMH was associated with higher cumulus granulosa cell TrkA mRNA. Both mural and cumulus granulosa cell p75 NTR/TrkA ratios were lower in women with higher serum AMH, and the number of oocytes retrieved was greater among women with low p75 NTR/TrkA ratio. No significant associations were found between brain-derived neurotrophic factor (BDNF) and its specific receptor tropomyosin-related kinase B (TrkB) and ovarian reserve markers. Although BDNF and TrkB expression were higher in cumulus compared with mural granulosa cells, no such association was found between TrkA and granulosa cells. Antimüllerian hormone and cumulus TrkA mRNA, in a model incorporating both, correlated strongly to the number of oocytes retrieved (R2 = 0.84). Conclusion(s): Cumulus TrkA and p75NTR mRNA correlate to ovarian reserve, whereas BDNF and TrkB are associated with the type of granulosa cell.

KW - antimüllerian hormone

KW - cumulus granulosa cell

KW - diminished ovarian reserve

KW - mural

KW - TrkA

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