Reduced expression of multiple forms of the alpha subunit of the stimulatory GTP-binding protein in pseudohypoparathyroidism type Ia.

A. Carter, C. Bardin, R. Collins, C. Simons, P. Bray, Allen M. Spiegel

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Abstract

We examined the expression of the alpha subunit of the stimulatory GTP-binding protein (Gs) in fibroblasts of subjects with pseudohypoparathyroidism (PHP) type Ia by transfer blot hybridization and S1 nuclease analyses. Six subjects with PHP type Ia showed decreased steady-state content of Gs alpha mRNA. S1 nuclease analysis indicates that both long and short forms of Gs alpha mRNA are decreased, with no apparent change in the ratio of long to short forms in PHP compared with normal individuals. It appears likely that in some cases of PHP type Ia the genetic lesion affects the maintenance of mRNA levels for all forms of the Gs alpha subunit.

Original languageEnglish (US)
Pages (from-to)7266-7269
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number20
StatePublished - Oct 1987
Externally publishedYes

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Gs GTP-Binding Protein alpha Subunits
Pseudohypoparathyroidism
Messenger RNA
Fibroblasts
Maintenance

ASJC Scopus subject areas

  • General
  • Genetics

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Reduced expression of multiple forms of the alpha subunit of the stimulatory GTP-binding protein in pseudohypoparathyroidism type Ia. / Carter, A.; Bardin, C.; Collins, R.; Simons, C.; Bray, P.; Spiegel, Allen M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 20, 10.1987, p. 7266-7269.

Research output: Contribution to journalArticle

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AU - Spiegel, Allen M.

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