Red fluorescent proteins: Chromophore formation and cellular applications

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

In the last decade, a number of red fluorescent proteins (RFPs) that emit orange, red, and far-red fluorescence have been isolated from anthozoans (corals), and developed through directed molecular evolution. An attractive property possessed by some RFPs is that their red fluorescence can be turned on or modulated by illumination at specific wavelengths. Recent progress in the development of RFPs has been accompanied with detailed studies of chromophore chemistry. A thorough understanding of the molecular mechanisms involved in the post-translational modifications of red chromophores would enable scientists to design RFPs with the desired properties to advance imaging applications. This article provides a broad perspective on the chemistry and applications of RFPs.

Original languageEnglish (US)
Pages (from-to)679-688
Number of pages10
JournalCurrent Opinion in Structural Biology
Volume22
Issue number5
DOIs
StatePublished - Oct 2012

Fingerprint

Directed Molecular Evolution
Fluorescence
Anthozoa
Post Translational Protein Processing
Lighting
red fluorescent protein

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Red fluorescent proteins : Chromophore formation and cellular applications. / Miyawaki, Atsushi; Shcherbakova, Daria; Verkhusha, Vladislav.

In: Current Opinion in Structural Biology, Vol. 22, No. 5, 10.2012, p. 679-688.

Research output: Contribution to journalArticle

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