Red fluorescent protein with reversibly photoswitchable absorbance for photochromic FRET

Fedor V. Subach, Lijuan Zhang, Theodorus W J Gadella, Nadya G. Gurskaya, Konstantin A. Lukyanov, Vladislav Verkhusha

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

We have developed the first red fluorescent protein, named rsTagRFP, which possesses reversibly photoswitchable absorbance spectra. Illumination with blue and yellow light switches rsTagRFP into a red fluorescent state (ON state) or nonfluorescent state (OFF state), respectively. The ON and OFF states exhibit absorbance maxima at 567 and 440 nm, respectively. Due to the photoswitchable absorbance, rsTagRFP can be used as an acceptor for a photochromic Förster resonance energy transfer (pcFRET). The photochromic acceptor facilitates determination of a protein-protein interaction by providing an internal control for FRET. Using pcFRET with EYFP as a donor, we observed an interaction between epidermal growth factor receptor and growth factor receptor-binding protein 2 in live cells by detecting the modulation of both the fluorescence intensity and lifetime of the EYFP donor upon the ON-OFF photoswitching of the rsTagRFP acceptor.

Original languageEnglish (US)
Pages (from-to)745-755
Number of pages11
JournalChemistry and Biology
Volume17
Issue number7
DOIs
StatePublished - 2010

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Energy Transfer
Energy transfer
Growth Factor Receptors
Lighting
Epidermal Growth Factor Receptor
Carrier Proteins
Proteins
Fluorescence
Switches
Modulation
Light
red fluorescent protein

Keywords

  • CHEMBIO

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Molecular Biology
  • Clinical Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Medicine(all)

Cite this

Red fluorescent protein with reversibly photoswitchable absorbance for photochromic FRET. / Subach, Fedor V.; Zhang, Lijuan; Gadella, Theodorus W J; Gurskaya, Nadya G.; Lukyanov, Konstantin A.; Verkhusha, Vladislav.

In: Chemistry and Biology, Vol. 17, No. 7, 2010, p. 745-755.

Research output: Contribution to journalArticle

Subach, Fedor V. ; Zhang, Lijuan ; Gadella, Theodorus W J ; Gurskaya, Nadya G. ; Lukyanov, Konstantin A. ; Verkhusha, Vladislav. / Red fluorescent protein with reversibly photoswitchable absorbance for photochromic FRET. In: Chemistry and Biology. 2010 ; Vol. 17, No. 7. pp. 745-755.
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AU - Lukyanov, Konstantin A.

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