Recruitment of PLANT U-BOX13 and the PI4Kβ1/β2 phosphatidylinositol-4 kinases by the small GTPase RabA4B plays important roles during salicylic acid-mediated plant defense signaling in arabidopsis

Vincenzo Antignani, Amy L. Klocko, Gwangbae Bak, Suma D. Chandrasekaran, Taylor Dunivin, Erik Nielsen

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Protection against microbial pathogens involves the activation of cellular immune responses in eukaryotes, and this cellular immunity likely involves changes in subcellular membrane trafficking. In eukaryotes, members of the Rab GTPase family of small monomeric regulatory GTPases play prominent roles in the regulation of membrane trafficking. We previously showed that RabA4B is recruited to vesicles that emerge from trans-Golgi network (TGN) compartments and regulates polarized membrane trafficking in plant cells. As part of this regulation, RabA4B recruits the closely related phosphatidylinositol 4-kinase (PI4K) PI4Kb1 and PI4Kb2 lipid kinases. Here, we identify a second Arabidopsis thaliana RabA4B-interacting protein, PLANT U-BOX13 (PUB13), which has recently been identified to play important roles in salicylic acid (SA)-mediated defense signaling. We show that PUB13 interacts with RabA4B through N-terminal domains and with phosphatidylinositol 4-phosphate (PI-4P) through a C-terminal armadillo domain. Furthermore, we demonstrate that a functional fluorescent PUB13 fusion protein (YFP-PUB13) localizes to TGN and Golgi compartments and that PUB13, PI4Kb1, and PI4Kb2 are negative regulators of SA-mediated induction of pathogenesis-related gene expression. Taken together, these results highlight a role for RabA4B and PI-4P in SA-dependent defense responses.

Original languageEnglish (US)
Pages (from-to)243-261
Number of pages19
JournalPlant Cell
Volume27
Issue number1
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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1-phosphatidylinositol 4-kinase
1-Phosphatidylinositol 4-Kinase
Monomeric GTP-Binding Proteins
Salicylic Acid
guanosinetriphosphatase
Arabidopsis
salicylic acid
trans-Golgi Network
phosphatidylinositols
Eukaryota
Cellular Immunity
cell-mediated immunity
Membranes
eukaryotic cells
rab GTP-Binding Proteins
phosphates
Armadillos
Dasypodidae
GTP Phosphohydrolases
Plant Cells

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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Recruitment of PLANT U-BOX13 and the PI4Kβ1/β2 phosphatidylinositol-4 kinases by the small GTPase RabA4B plays important roles during salicylic acid-mediated plant defense signaling in arabidopsis. / Antignani, Vincenzo; Klocko, Amy L.; Bak, Gwangbae; Chandrasekaran, Suma D.; Dunivin, Taylor; Nielsen, Erik.

In: Plant Cell, Vol. 27, No. 1, 01.01.2015, p. 243-261.

Research output: Contribution to journalArticle

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