Recording Synaptic Transmission from Auditory Mixed Synapses on the Mauthner Cells of Developing Zebrafish

Fabio A. Echeverry, Sundas Ijaz, Alberto E. Pereda

Research output: Contribution to journalArticlepeer-review

Abstract

The Mauthner cells are a pair of large reticulospinal neurons that organize sensory-evoked tail flip responses in fishes. An identifiable group of auditory “mixed” (electrical and chemical) synaptic contacts known as “Large Myelinated Club endings” on these cells have provided a valuable model for the study of synaptic transmission in the vertebrate brain. While most of studies were performed in adult fish, we describe here methods that make possible recording synaptic transmission from these contacts in developing zebrafish, a genetically tractable vertebrate species which is uniquely amenable for combining synaptic physiology with live imaging and behavioral analysis.

Original languageEnglish (US)
Article numberENEURO.0021-22.2022
JournaleNeuro
Volume9
Issue number3
DOIs
StatePublished - May 1 2022

Keywords

  • auditory afferent
  • connexin
  • electrical synapse
  • gap junction
  • glutamate
  • mixed transmission

ASJC Scopus subject areas

  • Neuroscience(all)

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