Abstract
Diphtheria toxin is a major factor of the pathogenicity of the causative agent of diphtheria Corynebacterium. Due to a small size, it is of considerable interest as the basis for the development of synthetic protein molecules with a transport function, e.g.; immunotoxins. In this work we describe the expression and characterized nontoxic recombinant fluorescent derivatives of the diphtheria toxin and its nontoxic CRM197 mutant. The proteins obtained can be used for studying receptor-binding and transport functions of the toxin in cells, evaluation of the expression level of the toxin proHB-EGF receptor membranes, immunization and generation of specific antibodies against the toxin, as well as for the development of diagnostic test-systems for the diphtheria toxin and antitoxic antibodies.
Original language | English (US) |
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Pages (from-to) | 401-409 |
Number of pages | 9 |
Journal | Russian Journal of Bioorganic Chemistry |
Volume | 40 |
Issue number | 4 |
DOIs | |
State | Published - Jun 2014 |
Keywords
- fluorescent proteins
- molecular cloning
- proHB-EGF
- recombinant nontoxic bacterial toxin analogues
ASJC Scopus subject areas
- Biochemistry
- Organic Chemistry