Recombinant fluorescent models for studying the diphtheria toxin

A. J. Labyntsev, N. V. Korotkevych, K. J. Manoilov, Andrii Kaberniuk, D. V. Kolybo, S. V. Komisarenko

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Diphtheria toxin is a major factor of the pathogenicity of the causative agent of diphtheria Corynebacterium. Due to a small size, it is of considerable interest as the basis for the development of synthetic protein molecules with a transport function, e.g.; immunotoxins. In this work we describe the expression and characterized nontoxic recombinant fluorescent derivatives of the diphtheria toxin and its nontoxic CRM197 mutant. The proteins obtained can be used for studying receptor-binding and transport functions of the toxin in cells, evaluation of the expression level of the toxin proHB-EGF receptor membranes, immunization and generation of specific antibodies against the toxin, as well as for the development of diagnostic test-systems for the diphtheria toxin and antitoxic antibodies.

Original languageEnglish (US)
Pages (from-to)401-409
Number of pages9
JournalRussian Journal of Bioorganic Chemistry
Volume40
Issue number4
DOIs
StatePublished - 2014

Fingerprint

Diphtheria Toxin
Corynebacterium diphtheriae
Immunization
Immunotoxins
Antibodies
Virulence Factors
Epidermal Growth Factor Receptor
Routine Diagnostic Tests
Proteins
Derivatives
Membranes
Molecules

Keywords

  • fluorescent proteins
  • molecular cloning
  • proHB-EGF
  • recombinant nontoxic bacterial toxin analogues

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry

Cite this

Labyntsev, A. J., Korotkevych, N. V., Manoilov, K. J., Kaberniuk, A., Kolybo, D. V., & Komisarenko, S. V. (2014). Recombinant fluorescent models for studying the diphtheria toxin. Russian Journal of Bioorganic Chemistry, 40(4), 401-409. https://doi.org/10.1134/S1068162014040086

Recombinant fluorescent models for studying the diphtheria toxin. / Labyntsev, A. J.; Korotkevych, N. V.; Manoilov, K. J.; Kaberniuk, Andrii; Kolybo, D. V.; Komisarenko, S. V.

In: Russian Journal of Bioorganic Chemistry, Vol. 40, No. 4, 2014, p. 401-409.

Research output: Contribution to journalArticle

Labyntsev, AJ, Korotkevych, NV, Manoilov, KJ, Kaberniuk, A, Kolybo, DV & Komisarenko, SV 2014, 'Recombinant fluorescent models for studying the diphtheria toxin', Russian Journal of Bioorganic Chemistry, vol. 40, no. 4, pp. 401-409. https://doi.org/10.1134/S1068162014040086
Labyntsev AJ, Korotkevych NV, Manoilov KJ, Kaberniuk A, Kolybo DV, Komisarenko SV. Recombinant fluorescent models for studying the diphtheria toxin. Russian Journal of Bioorganic Chemistry. 2014;40(4):401-409. https://doi.org/10.1134/S1068162014040086
Labyntsev, A. J. ; Korotkevych, N. V. ; Manoilov, K. J. ; Kaberniuk, Andrii ; Kolybo, D. V. ; Komisarenko, S. V. / Recombinant fluorescent models for studying the diphtheria toxin. In: Russian Journal of Bioorganic Chemistry. 2014 ; Vol. 40, No. 4. pp. 401-409.
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