Recombinant fluorescent models for studying the diphtheria toxin

A. J. Labyntsev, N. V. Korotkevych, K. J. Manoilov, A. A. Kaberniuk, D. V. Kolybo, S. V. Komisarenko

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Diphtheria toxin is a major factor of the pathogenicity of the causative agent of diphtheria Corynebacterium. Due to a small size, it is of considerable interest as the basis for the development of synthetic protein molecules with a transport function, e.g.; immunotoxins. In this work we describe the expression and characterized nontoxic recombinant fluorescent derivatives of the diphtheria toxin and its nontoxic CRM197 mutant. The proteins obtained can be used for studying receptor-binding and transport functions of the toxin in cells, evaluation of the expression level of the toxin proHB-EGF receptor membranes, immunization and generation of specific antibodies against the toxin, as well as for the development of diagnostic test-systems for the diphtheria toxin and antitoxic antibodies.

Original languageEnglish (US)
Pages (from-to)401-409
Number of pages9
JournalRussian Journal of Bioorganic Chemistry
Volume40
Issue number4
DOIs
StatePublished - Jun 2014

Keywords

  • fluorescent proteins
  • molecular cloning
  • proHB-EGF
  • recombinant nontoxic bacterial toxin analogues

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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  • Cite this

    Labyntsev, A. J., Korotkevych, N. V., Manoilov, K. J., Kaberniuk, A. A., Kolybo, D. V., & Komisarenko, S. V. (2014). Recombinant fluorescent models for studying the diphtheria toxin. Russian Journal of Bioorganic Chemistry, 40(4), 401-409. https://doi.org/10.1134/S1068162014040086