Reaction intermediates in the heme degradation reaction by HutZ from: Vibrio cholerae

Takeshi Uchida, Yukari Sekine, Nobuhiko Dojun, Ariel Lewis-Ballester, Izumi Ishigami, Toshitaka Matsui, Syun-Ru Yeh, Koichiro Ishimori

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

HutZ is a heme-degrading enzyme in Vibrio cholerae. It converts heme to biliverdin via verdoheme, suggesting that it follows the same reaction mechanism as that of mammalian heme oxygenase. However, none of the key intermediates have been identified. In this study, we applied steady-state and time-resolved UV-vis absorption and resonance Raman spectroscopy to study the reaction of the heme-HutZ complex with H2O2 or ascorbic acid. We characterized three intermediates: oxyferrous heme, meso-hydroxyheme, and verdoheme complexes. Our data support the view that HutZ degrades heme in a manner similar to mammalian heme oxygenase, despite their low sequence and structural homology.

Original languageEnglish (US)
Pages (from-to)8104-8109
Number of pages6
JournalDalton Transactions
Volume46
Issue number25
DOIs
StatePublished - 2017

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Reaction intermediates
Heme
Degradation
Heme Oxygenase (Decyclizing)
Biliverdine
Ascorbic Acid
Raman spectroscopy
Enzymes

ASJC Scopus subject areas

  • Inorganic Chemistry

Cite this

Uchida, T., Sekine, Y., Dojun, N., Lewis-Ballester, A., Ishigami, I., Matsui, T., ... Ishimori, K. (2017). Reaction intermediates in the heme degradation reaction by HutZ from: Vibrio cholerae. Dalton Transactions, 46(25), 8104-8109. https://doi.org/10.1039/c7dt01562c

Reaction intermediates in the heme degradation reaction by HutZ from : Vibrio cholerae. / Uchida, Takeshi; Sekine, Yukari; Dojun, Nobuhiko; Lewis-Ballester, Ariel; Ishigami, Izumi; Matsui, Toshitaka; Yeh, Syun-Ru; Ishimori, Koichiro.

In: Dalton Transactions, Vol. 46, No. 25, 2017, p. 8104-8109.

Research output: Contribution to journalArticle

Uchida, T, Sekine, Y, Dojun, N, Lewis-Ballester, A, Ishigami, I, Matsui, T, Yeh, S-R & Ishimori, K 2017, 'Reaction intermediates in the heme degradation reaction by HutZ from: Vibrio cholerae', Dalton Transactions, vol. 46, no. 25, pp. 8104-8109. https://doi.org/10.1039/c7dt01562c
Uchida, Takeshi ; Sekine, Yukari ; Dojun, Nobuhiko ; Lewis-Ballester, Ariel ; Ishigami, Izumi ; Matsui, Toshitaka ; Yeh, Syun-Ru ; Ishimori, Koichiro. / Reaction intermediates in the heme degradation reaction by HutZ from : Vibrio cholerae. In: Dalton Transactions. 2017 ; Vol. 46, No. 25. pp. 8104-8109.
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