Rational design of an improved tissue-engineered vascular graft: Determining the optimal cell dose and incubation time

Yong Ung Lee, Nathan Mahler, Cameron A. Best, Shuhei Tara, Tadahisa Sugiura, Avione Y. Lee, Tai Yi, Narutoshi Hibino, Toshiharu Shinoka, Christopher Breuer

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Aim: We investigated the effect of cell seeding dose and incubation time on tissue-engineered vascular graft (TEVG) patency. Materials & methods: Various doses of bone marrow-derived mononuclear cells (BM-MNCs) were seeded onto TEVGs, incubated for 0 or 12 h, and implanted in C57BL/6 mice. Different doses of human BM-MNCs were seeded onto TEVGs and measured for cell attachment. Results: The incubation time showed no significant effect on TEVG patency. However, TEVG patency was significantly increased in a dose-dependent manner. In the human graft, more bone marrow used for seeding resulted in increased cell attachment in a dose-dependent manner. Conclusion: Increasing the BM-MNC dose and reducing incubation time is a viable strategy for improving the performance and utility of the graft.

Original languageEnglish (US)
Pages (from-to)159-167
Number of pages9
JournalRegenerative Medicine
Volume11
Issue number2
DOIs
StatePublished - Mar 2016
Externally publishedYes

Keywords

  • BM-MNC
  • bone marrow mononuclear cells
  • C57BL/6 mice
  • congenital heart defect
  • Fontan operation
  • regenerative medicine
  • TEVG
  • tissue engineering

ASJC Scopus subject areas

  • Biomedical Engineering
  • Embryology

Fingerprint Dive into the research topics of 'Rational design of an improved tissue-engineered vascular graft: Determining the optimal cell dose and incubation time'. Together they form a unique fingerprint.

  • Cite this

    Lee, Y. U., Mahler, N., Best, C. A., Tara, S., Sugiura, T., Lee, A. Y., Yi, T., Hibino, N., Shinoka, T., & Breuer, C. (2016). Rational design of an improved tissue-engineered vascular graft: Determining the optimal cell dose and incubation time. Regenerative Medicine, 11(2), 159-167. https://doi.org/10.2217/rme.15.85