Abstract
Aim: We investigated the effect of cell seeding dose and incubation time on tissue-engineered vascular graft (TEVG) patency. Materials & methods: Various doses of bone marrow-derived mononuclear cells (BM-MNCs) were seeded onto TEVGs, incubated for 0 or 12 h, and implanted in C57BL/6 mice. Different doses of human BM-MNCs were seeded onto TEVGs and measured for cell attachment. Results: The incubation time showed no significant effect on TEVG patency. However, TEVG patency was significantly increased in a dose-dependent manner. In the human graft, more bone marrow used for seeding resulted in increased cell attachment in a dose-dependent manner. Conclusion: Increasing the BM-MNC dose and reducing incubation time is a viable strategy for improving the performance and utility of the graft.
Original language | English (US) |
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Pages (from-to) | 159-167 |
Number of pages | 9 |
Journal | Regenerative Medicine |
Volume | 11 |
Issue number | 2 |
DOIs | |
State | Published - Mar 2016 |
Externally published | Yes |
Keywords
- BM-MNC
- C57BL/6 mice
- Fontan operation
- TEVG
- bone marrow mononuclear cells
- congenital heart defect
- regenerative medicine
- tissue engineering
ASJC Scopus subject areas
- Biomedical Engineering
- Embryology