Rapid separation of low molecular weight solutes from liposomes without dilution

David W. Fry, J. Courtland White, I. David Goldman

Research output: Contribution to journalArticle

368 Scopus citations

Abstract

Liposomes can be separated from low molecular weight solutes on minicolumns of Sephadex G-50 made from the barrels of 1- or 5-ml plastic syringes. Excess fluid is first removed from the Sephadex beads by centrifugation and a mixture of liposomal entrapped and free solute is applied to the column bed. The centrifugation is repeated forcing the liposomal material through the column into a test tube while the free solute is quantitatively retained in the Sephadex. The procedure is applicable to a variety of solutes and 92 to 100% recovery is achieved for both charged and neutral liposomes. This technique has advantages over other methods for separating extraliposomal solutes from liposomes. Numerous samples can be processed simultaneously within minutes with no dilution of the liposomal preparation. Nonentrapped solute within the Sephadex can be easily recovered in a small volume of water or buffer.

Original languageEnglish (US)
Pages (from-to)809-815
Number of pages7
JournalAnalytical Biochemistry
Volume90
Issue number2
DOIs
StatePublished - Oct 15 1978
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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