Rapid mitochondrial DNA isolation method for direct sequencing

Wilber Quispe-Tintaya, Ryan R. White, Vasily N. Popov, Jan Vijg, Alexander Maslov

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

Standard methods for mitochondrial DNA (mtDNA) extraction do not provide the level of enrichment for mtDNA sufficient for direct sequencing and must be followed by long-range-PCR amplification, which can bias the sequence results. Here, we describe a reliable method for the preparation of mtDNA-enriched samples from eukaryotic cells ready for direct sequencing. This protocol utilizes a conventional miniprep kit, in conjunction with a paramagnetic bead-based purification step.

Original languageEnglish (US)
Title of host publicationProbing Mitochondrial Function
PublisherSpringer New York
Pages89-95
Number of pages7
Volume1
ISBN (Print)9781493922574, 9781493922567
DOIs
StatePublished - Jan 28 2015

Keywords

  • Mitochondrial DNA
  • Next-generation sequencing
  • Paramagnetic beads
  • Sequencing libraries
  • Solid-phase reversible immobilization

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Quispe-Tintaya, W., White, R. R., Popov, V. N., Vijg, J., & Maslov, A. (2015). Rapid mitochondrial DNA isolation method for direct sequencing. In Probing Mitochondrial Function (Vol. 1, pp. 89-95). Springer New York. https://doi.org/10.1007/978-1-4939-2257-4_9