Rapid mitochondrial DNA isolation method for direct sequencing

Wilber Quispe-tintaya, Ryan R. White, Vasily N. Popov, Jan Vijg, Alexander Y. Maslov

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Standard methods for mitochondrial DNA (mtDNA) extraction do not provide the level of enrichment for mtDNA suffi cient for direct sequencing and must be followed by long-range-PCR amplifi cation, which can bias the sequence results. Here, we describe a reliable method for the preparation of mtDNA-enriched samples from eukaryotic cells ready for direct sequencing. This protocol utilizes a conventional miniprep kit, in conjunction with a paramagnetic bead-based purifi cation step.

Original languageEnglish (US)
Pages (from-to)89-95
Number of pages7
JournalMethods in Molecular Biology
Volume1264
DOIs
StatePublished - 2015

Keywords

  • Mitochondrial DNA
  • Next-generation sequencing
  • Paramagnetic beads
  • Sequencing libraries
  • Solid-phase reversible immobilization

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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