Abstract
Standard methods for mitochondrial DNA (mtDNA) extraction do not provide the level of enrichment for mtDNA sufficient for direct sequencing and must be followed by long-range-PCR amplification, which can bias the sequence results. Here, we describe a reliable method for the preparation of mtDNA-enriched samples from eukaryotic cells ready for direct sequencing. This protocol utilizes a conventional miniprep kit, in conjunction with a paramagnetic bead-based purification step.
Original language | English (US) |
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Title of host publication | Probing Mitochondrial Function |
Publisher | Springer New York |
Pages | 89-95 |
Number of pages | 7 |
Volume | 1 |
ISBN (Print) | 9781493922574, 9781493922567 |
DOIs | |
State | Published - Jan 28 2015 |
Keywords
- Mitochondrial DNA
- Next-generation sequencing
- Paramagnetic beads
- Sequencing libraries
- Solid-phase reversible immobilization
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)