TY - JOUR
T1 - Rapid mitochondrial DNA isolation method for direct sequencing
AU - Quispe-tintaya, Wilber
AU - White, Ryan R.
AU - Popov, Vasily N.
AU - Vijg, Jan
AU - Maslov, Alexander Y.
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2015.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2015
Y1 - 2015
N2 - Standard methods for mitochondrial DNA (mtDNA) extraction do not provide the level of enrichment for mtDNA suffi cient for direct sequencing and must be followed by long-range-PCR amplifi cation, which can bias the sequence results. Here, we describe a reliable method for the preparation of mtDNA-enriched samples from eukaryotic cells ready for direct sequencing. This protocol utilizes a conventional miniprep kit, in conjunction with a paramagnetic bead-based purifi cation step.
AB - Standard methods for mitochondrial DNA (mtDNA) extraction do not provide the level of enrichment for mtDNA suffi cient for direct sequencing and must be followed by long-range-PCR amplifi cation, which can bias the sequence results. Here, we describe a reliable method for the preparation of mtDNA-enriched samples from eukaryotic cells ready for direct sequencing. This protocol utilizes a conventional miniprep kit, in conjunction with a paramagnetic bead-based purifi cation step.
KW - Mitochondrial DNA
KW - Next-generation sequencing
KW - Paramagnetic beads
KW - Sequencing libraries
KW - Solid-phase reversible immobilization
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U2 - 10.1007/978-1-4939-2257-4_9
DO - 10.1007/978-1-4939-2257-4_9
M3 - Article
C2 - 25631006
AN - SCOPUS:84958590781
VL - 1264
SP - 89
EP - 95
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -