Rapid mitochondrial DNA isolation method for direct sequencing

Wilber Quispe-Tintaya, Ryan R. White, Vasily N. Popov, Jan Vijg, Alexander Maslov

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Standard methods for mitochondrial DNA (mtDNA) extraction do not provide the level of enrichment for mtDNA sufficient for direct sequencing and must be followed by long-range-PCR amplification, which can bias the sequence results. Here, we describe a reliable method for the preparation of mtDNA-enriched samples from eukaryotic cells ready for direct sequencing. This protocol utilizes a conventional miniprep kit, in conjunction with a paramagnetic bead-based purification step.

Original languageEnglish (US)
Title of host publicationProbing Mitochondrial Function
PublisherSpringer New York
Pages89-95
Number of pages7
Volume1
ISBN (Print)9781493922574, 9781493922567
DOIs
StatePublished - Jan 28 2015

Fingerprint

Mitochondrial DNA
Eukaryotic Cells
Purification
Amplification
Polymerase Chain Reaction

Keywords

  • Mitochondrial DNA
  • Next-generation sequencing
  • Paramagnetic beads
  • Sequencing libraries
  • Solid-phase reversible immobilization

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Quispe-Tintaya, W., White, R. R., Popov, V. N., Vijg, J., & Maslov, A. (2015). Rapid mitochondrial DNA isolation method for direct sequencing. In Probing Mitochondrial Function (Vol. 1, pp. 89-95). Springer New York. https://doi.org/10.1007/978-1-4939-2257-4_9

Rapid mitochondrial DNA isolation method for direct sequencing. / Quispe-Tintaya, Wilber; White, Ryan R.; Popov, Vasily N.; Vijg, Jan; Maslov, Alexander.

Probing Mitochondrial Function. Vol. 1 Springer New York, 2015. p. 89-95.

Research output: Chapter in Book/Report/Conference proceedingChapter

Quispe-Tintaya, W, White, RR, Popov, VN, Vijg, J & Maslov, A 2015, Rapid mitochondrial DNA isolation method for direct sequencing. in Probing Mitochondrial Function. vol. 1, Springer New York, pp. 89-95. https://doi.org/10.1007/978-1-4939-2257-4_9
Quispe-Tintaya W, White RR, Popov VN, Vijg J, Maslov A. Rapid mitochondrial DNA isolation method for direct sequencing. In Probing Mitochondrial Function. Vol. 1. Springer New York. 2015. p. 89-95 https://doi.org/10.1007/978-1-4939-2257-4_9
Quispe-Tintaya, Wilber ; White, Ryan R. ; Popov, Vasily N. ; Vijg, Jan ; Maslov, Alexander. / Rapid mitochondrial DNA isolation method for direct sequencing. Probing Mitochondrial Function. Vol. 1 Springer New York, 2015. pp. 89-95
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