Rapid mitochondrial DNA isolation method for direct sequencing

Wilber Quispe-Tintaya, Ryan R. White, Vasily N. Popov, Jan Vijg, Alexander Y. Maslov

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Standard methods for mitochondrial DNA (mtDNA) extraction do not provide the level of enrichment for mtDNA sufficient for direct sequencing and must be followed by long-range-PCR amplification, which can bias the sequence results. Here, we describe a reliable method for the preparation of mtDNA-enriched samples from eukaryotic cells ready for direct sequencing. This protocol utilizes a conventional miniprep kit, in conjunction with a paramagnetic bead-based purification step.

Original languageEnglish (US)
Pages (from-to)89-95
Number of pages7
JournalMethods in molecular biology (Clifton, N.J.)
Volume1264
DOIs
StatePublished - Jan 1 2015

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Rapid mitochondrial DNA isolation method for direct sequencing'. Together they form a unique fingerprint.

Cite this