Rapid cloning of rearranged immunoglobulin heavy chain genes from human B-cell lines using anchored polymerase chain reaction

Howard Ratech

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A general method to obtain the variable region DNA sequence of the immunoglobulin heavy chain using anchored polymerase chain reaction is described. Based on this DNA sequence, clone-specific oligonucleotides were designed to anneal to the complentarity determining regions. These were used to identify the original B-cell clone in serial dilutions of polyclonal lymph node DNA with high specificity and sensitivity. This method should be useful for studying minimal residual disease in B-cell neoplasia.

Original languageEnglish (US)
Pages (from-to)1260-1263
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume182
Issue number3
DOIs
StatePublished - Feb 14 1992
Externally publishedYes

Fingerprint

Immunoglobulin Heavy Chain Genes
Immunoglobulin Heavy Chains
Cloning
Polymerase chain reaction
DNA sequences
Organism Cloning
Clone cells
B-Lymphocytes
Clone Cells
Genes
Cells
Cell Line
Polymerase Chain Reaction
Residual Neoplasm
Oligonucleotides
Dilution
Lymph Nodes
Sensitivity and Specificity
DNA
Neoplasms

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Rapid cloning of rearranged immunoglobulin heavy chain genes from human B-cell lines using anchored polymerase chain reaction. / Ratech, Howard.

In: Biochemical and Biophysical Research Communications, Vol. 182, No. 3, 14.02.1992, p. 1260-1263.

Research output: Contribution to journalArticle

@article{42c567ffd12f4acd832d29e41f9e83cc,
title = "Rapid cloning of rearranged immunoglobulin heavy chain genes from human B-cell lines using anchored polymerase chain reaction",
abstract = "A general method to obtain the variable region DNA sequence of the immunoglobulin heavy chain using anchored polymerase chain reaction is described. Based on this DNA sequence, clone-specific oligonucleotides were designed to anneal to the complentarity determining regions. These were used to identify the original B-cell clone in serial dilutions of polyclonal lymph node DNA with high specificity and sensitivity. This method should be useful for studying minimal residual disease in B-cell neoplasia.",
author = "Howard Ratech",
year = "1992",
month = "2",
day = "14",
doi = "10.1016/0006-291X(92)91867-P",
language = "English (US)",
volume = "182",
pages = "1260--1263",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Rapid cloning of rearranged immunoglobulin heavy chain genes from human B-cell lines using anchored polymerase chain reaction

AU - Ratech, Howard

PY - 1992/2/14

Y1 - 1992/2/14

N2 - A general method to obtain the variable region DNA sequence of the immunoglobulin heavy chain using anchored polymerase chain reaction is described. Based on this DNA sequence, clone-specific oligonucleotides were designed to anneal to the complentarity determining regions. These were used to identify the original B-cell clone in serial dilutions of polyclonal lymph node DNA with high specificity and sensitivity. This method should be useful for studying minimal residual disease in B-cell neoplasia.

AB - A general method to obtain the variable region DNA sequence of the immunoglobulin heavy chain using anchored polymerase chain reaction is described. Based on this DNA sequence, clone-specific oligonucleotides were designed to anneal to the complentarity determining regions. These were used to identify the original B-cell clone in serial dilutions of polyclonal lymph node DNA with high specificity and sensitivity. This method should be useful for studying minimal residual disease in B-cell neoplasia.

UR - http://www.scopus.com/inward/record.url?scp=0026554228&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026554228&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(92)91867-P

DO - 10.1016/0006-291X(92)91867-P

M3 - Article

C2 - 1540170

AN - SCOPUS:0026554228

VL - 182

SP - 1260

EP - 1263

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -