Rapid cloning of rearranged immunoglobulin heavy chain genes from human B-cell lines using anchored polymerase chain reaction

Howard Ratech

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A general method to obtain the variable region DNA sequence of the immunoglobulin heavy chain using anchored polymerase chain reaction is described. Based on this DNA sequence, clone-specific oligonucleotides were designed to anneal to the complentarity determining regions. These were used to identify the original B-cell clone in serial dilutions of polyclonal lymph node DNA with high specificity and sensitivity. This method should be useful for studying minimal residual disease in B-cell neoplasia.

Original languageEnglish (US)
Pages (from-to)1260-1263
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume182
Issue number3
DOIs
Publication statusPublished - Feb 14 1992

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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