Rapid cloning of rearranged immunoglobulin heavy chain genes from human B-cell lines using anchored polymerase chain reaction

Howard Ratech

doi:10.1016/0006-291X(92)91867-P

Rapid cloning of rearranged immunoglobulin heavy chain genes from human B-cell lines using anchored polymerase chain reaction

Howard Ratech

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A general method to obtain the variable region DNA sequence of the immunoglobulin heavy chain using anchored polymerase chain reaction is described. Based on this DNA sequence, clone-specific oligonucleotides were designed to anneal to the complentarity determining regions. These were used to identify the original B-cell clone in serial dilutions of polyclonal lymph node DNA with high specificity and sensitivity. This method should be useful for studying minimal residual disease in B-cell neoplasia.

Original language	English (US)
Pages (from-to)	1260-1263
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	182
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(92)91867-P
State	Published - Feb 14 1992
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(92)91867-P

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abstract = "A general method to obtain the variable region DNA sequence of the immunoglobulin heavy chain using anchored polymerase chain reaction is described. Based on this DNA sequence, clone-specific oligonucleotides were designed to anneal to the complentarity determining regions. These were used to identify the original B-cell clone in serial dilutions of polyclonal lymph node DNA with high specificity and sensitivity. This method should be useful for studying minimal residual disease in B-cell neoplasia.",

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N2 - A general method to obtain the variable region DNA sequence of the immunoglobulin heavy chain using anchored polymerase chain reaction is described. Based on this DNA sequence, clone-specific oligonucleotides were designed to anneal to the complentarity determining regions. These were used to identify the original B-cell clone in serial dilutions of polyclonal lymph node DNA with high specificity and sensitivity. This method should be useful for studying minimal residual disease in B-cell neoplasia.

AB - A general method to obtain the variable region DNA sequence of the immunoglobulin heavy chain using anchored polymerase chain reaction is described. Based on this DNA sequence, clone-specific oligonucleotides were designed to anneal to the complentarity determining regions. These were used to identify the original B-cell clone in serial dilutions of polyclonal lymph node DNA with high specificity and sensitivity. This method should be useful for studying minimal residual disease in B-cell neoplasia.

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U2 - 10.1016/0006-291X(92)91867-P

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ER -

Rapid cloning of rearranged immunoglobulin heavy chain genes from human B-cell lines using anchored polymerase chain reaction

Abstract

ASJC Scopus subject areas

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