Rapid assay for cyclic AMP and cyclic GMP phosphodiesterases

Rafael Rangel-Aldao, David Schwartz, Charles S. Rubin

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

A rapid highly sensitive assay for cyclic AMP phosphodiesterase has been devised. After a 5-min incubation, cyclic AMP is readily resolved from 5′-AMP, adenosine, and inosine by ion-exchange thin-layer chromatography on 1.3 × 6.5-cm strips of PEI-cellulose for 7 to 8 min. This procedure combines the accuracy of the standard paper chromatography assay (1) with the speed of ion-exchange resin techniques (2), while surmounting some of the major drawbacks of the other two methods (3). Since chromatography on PEI-cellulose efficiently resolves cyclic GMP, 5′-GMP, and guanosine, this methodology has also been adapted to the measurement of cyclic GMP hydrolysis.

Original languageEnglish (US)
Pages (from-to)367-375
Number of pages9
JournalAnalytical Biochemistry
Volume87
Issue number2
DOIs
StatePublished - Jul 1 1978

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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