Rapid assay for cyclic AMP and cyclic GMP phosphodiesterases

Rafael Rangel-Aldao, David Schwartz, Charles S. Rubin

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A rapid highly sensitive assay for cyclic AMP phosphodiesterase has been devised. After a 5-min incubation, cyclic AMP is readily resolved from 5′-AMP, adenosine, and inosine by ion-exchange thin-layer chromatography on 1.3 × 6.5-cm strips of PEI-cellulose for 7 to 8 min. This procedure combines the accuracy of the standard paper chromatography assay (1) with the speed of ion-exchange resin techniques (2), while surmounting some of the major drawbacks of the other two methods (3). Since chromatography on PEI-cellulose efficiently resolves cyclic GMP, 5′-GMP, and guanosine, this methodology has also been adapted to the measurement of cyclic GMP hydrolysis.

Original languageEnglish (US)
Pages (from-to)367-375
Number of pages9
JournalAnalytical Biochemistry
Volume87
Issue number2
DOIs
StatePublished - Jul 1 1978

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Polyetherimides
Cyclic GMP
Phosphoric Diester Hydrolases
Chromatography
Cellulose
Cyclic AMP
Assays
Ion Exchange Resins
Guanosine Monophosphate
Thin layer chromatography
Paper Chromatography
Inosine
Guanosine
Ion Exchange Chromatography
Adenosine Monophosphate
Thin Layer Chromatography
Adenosine
Hydrolysis
Ion exchange

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Rapid assay for cyclic AMP and cyclic GMP phosphodiesterases. / Rangel-Aldao, Rafael; Schwartz, David; Rubin, Charles S.

In: Analytical Biochemistry, Vol. 87, No. 2, 01.07.1978, p. 367-375.

Research output: Contribution to journalArticle

Rangel-Aldao, Rafael ; Schwartz, David ; Rubin, Charles S. / Rapid assay for cyclic AMP and cyclic GMP phosphodiesterases. In: Analytical Biochemistry. 1978 ; Vol. 87, No. 2. pp. 367-375.
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