TY - JOUR
T1 - Rapid assay for cyclic AMP and cyclic GMP phosphodiesterases
AU - Rangel-Aldao, Rafael
AU - Schwartz, David
AU - Rubin, Charles S.
N1 - Funding Information:
This research was supported by Grant GM22792 from the National Institutes Charles S. Rubin is the recipient of a Research Career Development Award (K04 from the National Institutes of Health.
PY - 1978/7/1
Y1 - 1978/7/1
N2 - A rapid highly sensitive assay for cyclic AMP phosphodiesterase has been devised. After a 5-min incubation, cyclic AMP is readily resolved from 5′-AMP, adenosine, and inosine by ion-exchange thin-layer chromatography on 1.3 × 6.5-cm strips of PEI-cellulose for 7 to 8 min. This procedure combines the accuracy of the standard paper chromatography assay (1) with the speed of ion-exchange resin techniques (2), while surmounting some of the major drawbacks of the other two methods (3). Since chromatography on PEI-cellulose efficiently resolves cyclic GMP, 5′-GMP, and guanosine, this methodology has also been adapted to the measurement of cyclic GMP hydrolysis.
AB - A rapid highly sensitive assay for cyclic AMP phosphodiesterase has been devised. After a 5-min incubation, cyclic AMP is readily resolved from 5′-AMP, adenosine, and inosine by ion-exchange thin-layer chromatography on 1.3 × 6.5-cm strips of PEI-cellulose for 7 to 8 min. This procedure combines the accuracy of the standard paper chromatography assay (1) with the speed of ion-exchange resin techniques (2), while surmounting some of the major drawbacks of the other two methods (3). Since chromatography on PEI-cellulose efficiently resolves cyclic GMP, 5′-GMP, and guanosine, this methodology has also been adapted to the measurement of cyclic GMP hydrolysis.
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U2 - 10.1016/0003-2697(78)90686-3
DO - 10.1016/0003-2697(78)90686-3
M3 - Article
C2 - 210688
AN - SCOPUS:0018080954
SN - 0003-2697
VL - 87
SP - 367
EP - 375
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -