Rapid accumulation of genome rearrangements in liver but not in brain of old mice

Martijn E T Dollé, Heidi Giese, Craig L. Hopkins, Hans Jörg Martus, Jeffrey M. Hausdorff, Jan Vijg

Research output: Contribution to journalArticle

199 Citations (Scopus)

Abstract

Somatic mutations have long been considered a possible cause of ageing. To directly study mutational events in organs and tissues of ageing mammals, a transgenic mouse model has been generated that harbours lacZ reporter genes as part of chromosomally integrated plasmids. Using this model, we determined spontaneous mutant frequencies and spectra in mouse liver end brain as a function of age. In the liver, mutant frequencies increased with age from birth to 34 months; in the brain, an increase was observed only between birth end 4-6 months. Molecular characterization of the mutations showed that a substantial portion involved genome rearrangement events, with one breakpoint in e reporter gene and the other in the mouse flanking sequence. In the liver, these genome rearrangements did not increase with age until after 27 months, when they increased rapidly. In brain, the frequency of genome rearrangements was lower than in liver and did not increase with age.

Original languageEnglish (US)
Pages (from-to)431-434
Number of pages4
JournalNature Genetics
Volume17
Issue number4
StatePublished - 1997
Externally publishedYes

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Genome
Liver
Brain
Reporter Genes
Parturition
Mutation
Lac Operon
Transgenic Mice
Mammals
Plasmids

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Dollé, M. E. T., Giese, H., Hopkins, C. L., Martus, H. J., Hausdorff, J. M., & Vijg, J. (1997). Rapid accumulation of genome rearrangements in liver but not in brain of old mice. Nature Genetics, 17(4), 431-434.

Rapid accumulation of genome rearrangements in liver but not in brain of old mice. / Dollé, Martijn E T; Giese, Heidi; Hopkins, Craig L.; Martus, Hans Jörg; Hausdorff, Jeffrey M.; Vijg, Jan.

In: Nature Genetics, Vol. 17, No. 4, 1997, p. 431-434.

Research output: Contribution to journalArticle

Dollé, MET, Giese, H, Hopkins, CL, Martus, HJ, Hausdorff, JM & Vijg, J 1997, 'Rapid accumulation of genome rearrangements in liver but not in brain of old mice', Nature Genetics, vol. 17, no. 4, pp. 431-434.
Dollé MET, Giese H, Hopkins CL, Martus HJ, Hausdorff JM, Vijg J. Rapid accumulation of genome rearrangements in liver but not in brain of old mice. Nature Genetics. 1997;17(4):431-434.
Dollé, Martijn E T ; Giese, Heidi ; Hopkins, Craig L. ; Martus, Hans Jörg ; Hausdorff, Jeffrey M. ; Vijg, Jan. / Rapid accumulation of genome rearrangements in liver but not in brain of old mice. In: Nature Genetics. 1997 ; Vol. 17, No. 4. pp. 431-434.
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