Rap1 activation in collagen phagocytosis is dependent on nonmuscle myosin II-A

Pamela D. Arora, Mary Anne Conti, Shoshana Ravid, David B. Sacks, Andras Kapus, Robert S. Adelstein, Anne R. Bresnick, Christopher A. McCulloch

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Rap1 enhances integrin-mediated adhesion but the link between Rap1 activation and integrin function in collagen phagocytosis is not defined. Mass spectrometry of Rap1 immunoprecipitates showed that the association of Rap1 with nonmuscle myosin heavy-chain II-A (NMHC II-A) was enhanced by cell attachment to collagen beads. Rap1 colocalized with NM II-A at collagen bead-binding sites. There was a transient increase in myosin light-chain phosphorylation after collagen-bead binding that was dependent on myosin light-chain kinase but not Rho kinase. Inhibition of myosin light-chain phosphorylation, but not myosin II-A motor activity inhibited collagen-bead binding and Rap activation. In vitro binding assays demonstrated binding of Rap1A to filamentous myosin rods, and in situ staining of permeabilized cells showed that NM II-A filaments colocalized with F-actin at collagen bead sites. Knockdown of NM II-A did not affect talin, actin, or β1-integrin targeting to collagen beads but targeting of Rap1 and vinculin to collagen was inhibited. Conversely, knockdown of Rap1 did not affect localization of NM II-A to beads. We conclude that MLC phosphorylation in response to initial collagen-bead binding promotes NM II-A filament assembly; binding of Rap1 to myosin filaments enables Rap1-dependent integrin activation and enhanced collagen phagocytosis.

Original languageEnglish (US)
Pages (from-to)5032-5046
Number of pages15
JournalMolecular Biology of the Cell
Volume19
Issue number12
DOIs
StatePublished - Dec 2008

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Myosin Type II
Phagocytosis
Collagen
Integrins
Myosin Light Chains
Phosphorylation
Actins
Talin
Vinculin
Myosin-Light-Chain Kinase
Myosin Subfragments
rho-Associated Kinases
Myosin Heavy Chains
Myosins
Mass Spectrometry
Motor Activity
Binding Sites
Staining and Labeling

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Arora, P. D., Conti, M. A., Ravid, S., Sacks, D. B., Kapus, A., Adelstein, R. S., ... McCulloch, C. A. (2008). Rap1 activation in collagen phagocytosis is dependent on nonmuscle myosin II-A. Molecular Biology of the Cell, 19(12), 5032-5046. https://doi.org/10.1091/mbc.E08-04-0430

Rap1 activation in collagen phagocytosis is dependent on nonmuscle myosin II-A. / Arora, Pamela D.; Conti, Mary Anne; Ravid, Shoshana; Sacks, David B.; Kapus, Andras; Adelstein, Robert S.; Bresnick, Anne R.; McCulloch, Christopher A.

In: Molecular Biology of the Cell, Vol. 19, No. 12, 12.2008, p. 5032-5046.

Research output: Contribution to journalArticle

Arora, PD, Conti, MA, Ravid, S, Sacks, DB, Kapus, A, Adelstein, RS, Bresnick, AR & McCulloch, CA 2008, 'Rap1 activation in collagen phagocytosis is dependent on nonmuscle myosin II-A', Molecular Biology of the Cell, vol. 19, no. 12, pp. 5032-5046. https://doi.org/10.1091/mbc.E08-04-0430
Arora PD, Conti MA, Ravid S, Sacks DB, Kapus A, Adelstein RS et al. Rap1 activation in collagen phagocytosis is dependent on nonmuscle myosin II-A. Molecular Biology of the Cell. 2008 Dec;19(12):5032-5046. https://doi.org/10.1091/mbc.E08-04-0430
Arora, Pamela D. ; Conti, Mary Anne ; Ravid, Shoshana ; Sacks, David B. ; Kapus, Andras ; Adelstein, Robert S. ; Bresnick, Anne R. ; McCulloch, Christopher A. / Rap1 activation in collagen phagocytosis is dependent on nonmuscle myosin II-A. In: Molecular Biology of the Cell. 2008 ; Vol. 19, No. 12. pp. 5032-5046.
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