Raman study of reduced nicotinamide adenine dinucleotide bound to liver alcohol dehydrogenase

Kwok To Yue, Jing Peng Yang, Charlotte L. Martin, Robert Callender, Donald L. Sloan, Robert H. Callender

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We report the first Raman spectra of reduced nicotinamide adenine dinucleotide (NADH) when bound to an enzymatic active site, that of liver alcohol dehydrogenase (LADH). This was obtained by subtracting the Raman spectrum of LADH from that of the binary LADH/NADH complex. There are significant changes in the spectrum of bound NADH as compared to that in solution. The data indicate that both the nicotinamide moiety and the adenine moiety are involved in the binding. At least one of the two NH2 moieties of NADH also participates.

Original languageEnglish (US)
Pages (from-to)6480-6483
Number of pages4
JournalBiochemistry
Volume23
Issue number26
StatePublished - 1984
Externally publishedYes

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Alcohol Dehydrogenase
Liver
NAD
Raman scattering
Niacinamide
Adenine
Catalytic Domain

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yue, K. T., Yang, J. P., Martin, C. L., Callender, R., Sloan, D. L., & Callender, R. H. (1984). Raman study of reduced nicotinamide adenine dinucleotide bound to liver alcohol dehydrogenase. Biochemistry, 23(26), 6480-6483.

Raman study of reduced nicotinamide adenine dinucleotide bound to liver alcohol dehydrogenase. / Yue, Kwok To; Yang, Jing Peng; Martin, Charlotte L.; Callender, Robert; Sloan, Donald L.; Callender, Robert H.

In: Biochemistry, Vol. 23, No. 26, 1984, p. 6480-6483.

Research output: Contribution to journalArticle

Yue, KT, Yang, JP, Martin, CL, Callender, R, Sloan, DL & Callender, RH 1984, 'Raman study of reduced nicotinamide adenine dinucleotide bound to liver alcohol dehydrogenase', Biochemistry, vol. 23, no. 26, pp. 6480-6483.
Yue, Kwok To ; Yang, Jing Peng ; Martin, Charlotte L. ; Callender, Robert ; Sloan, Donald L. ; Callender, Robert H. / Raman study of reduced nicotinamide adenine dinucleotide bound to liver alcohol dehydrogenase. In: Biochemistry. 1984 ; Vol. 23, No. 26. pp. 6480-6483.
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