Raman spectroscopy of oxidized and reduced nicotinamide adenine dinucleotides

Kwok To Yue, Charlotte L. Martin, Dehuai Chen, Paula Nelson, Donald L. Sloan, Robert Callender

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

We have measured the Raman spectra of oxidized nicotinamide adenine dinucleotide, NAD+, and its reduced form, NADH, as well as a series of fragments and analogues of NAD+ and NADH. In addition, we have studied the effects of pH as well as deuteration of the exchangable protons on the Raman spectra of these molecules. In comparing the positions and intensities of the peaks in the fragment and analogue spectra with those of NADH and NAD+, we find that it is useful to consider these large molecules as consisting of component parts, namely, adenine, two ribose groups, two phosphate groups, and nicotinamide, for the purpose of assigning their spectral features. The Raman bands of NADH and NAD+ are found generally to arise from molecular motions in one or another of these molecular moieties, although some peaks are not quite so easily identified in this way. This type of assignment is the first step in a detailed understanding of the Raman spectra of NAD+ and NADH. This is needed to understand the binding properties of NADH and NAD+ acting as coenzymes with the NAD-linked dehydrogenases as deduced recently by using Raman spectroscopy.

Original languageEnglish (US)
Pages (from-to)4941-4947
Number of pages7
JournalBiochemistry®
Volume25
Issue number17
StatePublished - 1986
Externally publishedYes

Fingerprint

Raman Spectrum Analysis
NAD
Raman spectroscopy
Raman scattering
Molecules
Ribose
Niacinamide
Coenzymes
Adenine
Protons
Oxidoreductases

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yue, K. T., Martin, C. L., Chen, D., Nelson, P., Sloan, D. L., & Callender, R. (1986). Raman spectroscopy of oxidized and reduced nicotinamide adenine dinucleotides. Biochemistry®, 25(17), 4941-4947.

Raman spectroscopy of oxidized and reduced nicotinamide adenine dinucleotides. / Yue, Kwok To; Martin, Charlotte L.; Chen, Dehuai; Nelson, Paula; Sloan, Donald L.; Callender, Robert.

In: Biochemistry®, Vol. 25, No. 17, 1986, p. 4941-4947.

Research output: Contribution to journalArticle

Yue, KT, Martin, CL, Chen, D, Nelson, P, Sloan, DL & Callender, R 1986, 'Raman spectroscopy of oxidized and reduced nicotinamide adenine dinucleotides', Biochemistry®, vol. 25, no. 17, pp. 4941-4947.
Yue KT, Martin CL, Chen D, Nelson P, Sloan DL, Callender R. Raman spectroscopy of oxidized and reduced nicotinamide adenine dinucleotides. Biochemistry®. 1986;25(17):4941-4947.
Yue, Kwok To ; Martin, Charlotte L. ; Chen, Dehuai ; Nelson, Paula ; Sloan, Donald L. ; Callender, Robert. / Raman spectroscopy of oxidized and reduced nicotinamide adenine dinucleotides. In: Biochemistry®. 1986 ; Vol. 25, No. 17. pp. 4941-4947.
@article{ccfed23c927c4ff8914ad1c275a10f54,
title = "Raman spectroscopy of oxidized and reduced nicotinamide adenine dinucleotides",
abstract = "We have measured the Raman spectra of oxidized nicotinamide adenine dinucleotide, NAD+, and its reduced form, NADH, as well as a series of fragments and analogues of NAD+ and NADH. In addition, we have studied the effects of pH as well as deuteration of the exchangable protons on the Raman spectra of these molecules. In comparing the positions and intensities of the peaks in the fragment and analogue spectra with those of NADH and NAD+, we find that it is useful to consider these large molecules as consisting of component parts, namely, adenine, two ribose groups, two phosphate groups, and nicotinamide, for the purpose of assigning their spectral features. The Raman bands of NADH and NAD+ are found generally to arise from molecular motions in one or another of these molecular moieties, although some peaks are not quite so easily identified in this way. This type of assignment is the first step in a detailed understanding of the Raman spectra of NAD+ and NADH. This is needed to understand the binding properties of NADH and NAD+ acting as coenzymes with the NAD-linked dehydrogenases as deduced recently by using Raman spectroscopy.",
author = "Yue, {Kwok To} and Martin, {Charlotte L.} and Dehuai Chen and Paula Nelson and Sloan, {Donald L.} and Robert Callender",
year = "1986",
language = "English (US)",
volume = "25",
pages = "4941--4947",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "17",

}

TY - JOUR

T1 - Raman spectroscopy of oxidized and reduced nicotinamide adenine dinucleotides

AU - Yue, Kwok To

AU - Martin, Charlotte L.

AU - Chen, Dehuai

AU - Nelson, Paula

AU - Sloan, Donald L.

AU - Callender, Robert

PY - 1986

Y1 - 1986

N2 - We have measured the Raman spectra of oxidized nicotinamide adenine dinucleotide, NAD+, and its reduced form, NADH, as well as a series of fragments and analogues of NAD+ and NADH. In addition, we have studied the effects of pH as well as deuteration of the exchangable protons on the Raman spectra of these molecules. In comparing the positions and intensities of the peaks in the fragment and analogue spectra with those of NADH and NAD+, we find that it is useful to consider these large molecules as consisting of component parts, namely, adenine, two ribose groups, two phosphate groups, and nicotinamide, for the purpose of assigning their spectral features. The Raman bands of NADH and NAD+ are found generally to arise from molecular motions in one or another of these molecular moieties, although some peaks are not quite so easily identified in this way. This type of assignment is the first step in a detailed understanding of the Raman spectra of NAD+ and NADH. This is needed to understand the binding properties of NADH and NAD+ acting as coenzymes with the NAD-linked dehydrogenases as deduced recently by using Raman spectroscopy.

AB - We have measured the Raman spectra of oxidized nicotinamide adenine dinucleotide, NAD+, and its reduced form, NADH, as well as a series of fragments and analogues of NAD+ and NADH. In addition, we have studied the effects of pH as well as deuteration of the exchangable protons on the Raman spectra of these molecules. In comparing the positions and intensities of the peaks in the fragment and analogue spectra with those of NADH and NAD+, we find that it is useful to consider these large molecules as consisting of component parts, namely, adenine, two ribose groups, two phosphate groups, and nicotinamide, for the purpose of assigning their spectral features. The Raman bands of NADH and NAD+ are found generally to arise from molecular motions in one or another of these molecular moieties, although some peaks are not quite so easily identified in this way. This type of assignment is the first step in a detailed understanding of the Raman spectra of NAD+ and NADH. This is needed to understand the binding properties of NADH and NAD+ acting as coenzymes with the NAD-linked dehydrogenases as deduced recently by using Raman spectroscopy.

UR - http://www.scopus.com/inward/record.url?scp=0022551940&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022551940&partnerID=8YFLogxK

M3 - Article

C2 - 3768324

AN - SCOPUS:0022551940

VL - 25

SP - 4941

EP - 4947

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 17

ER -