RAG-1 mutations that affect the target specificity of V(D)J recombination: A possible direct role of RAG-1 in site recognition

Moshe J. Sadofsky, Joanne E. Hesse, Dik C. Van Gent, Martin Gellert

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

The RAG-1 protein plays an essential role in V(D)J recombination, but its exact function has not yet been defined. Here we report that a particular mutation in RAG-1 affects recombination by altering the specificity of target sequence usage. Recombination mediated by wild-type RAG-1 is tolerant of a wide range of coding sequences adjacent to the recombination signal. With the mutant RAG-1, recombination is much more demanding; efficient recombination is only found when particular dinucleotides are adjacent to the signal sequence heptamer. The mutant is also more sensitive than wild-type RAG-1 to certain alterations within the signal sequence. We suggest that the RAG-1 protein may interact physically with the target DNA at the coding-signal sequence border.

Original languageEnglish (US)
Pages (from-to)2193-2199
Number of pages7
JournalGenes and Development
Volume9
Issue number17
DOIs
Publication statusPublished - Sep 1 1995
Externally publishedYes

    Fingerprint

Keywords

  • RAG-1
  • V(D)J recombination
  • mutagenesis
  • plasmid library

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this