RAG-1 mutations that affect the target specificity of V(D)J recombination

A possible direct role of RAG-1 in site recognition

Moshe J. Sadofsky, J. E. Hesse, D. C. Van Gent, M. Gellert

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The RAG-1 protein plays an essential role in V(D)J recombination, but its exact function has not yet been defined. Here we report that a particular mutation in RAG-1 affects recombination by altering the specificity of target sequence usage. Recombination mediated by wild-type RAG-1 is tolerant of a wide range of coding sequences adjacent to the recombination signal. With the mutant RAG-1, recombination is much more demanding; efficient recombination is only found when particular dinucleotides are adjacent to the signal sequence heptamer. The mutant is also more sensitive than wild-type RAG-1 to certain alterations within the signal sequence. We suggest that the RAG-1 protein may interact physically with the target DNA at the coding-signal sequence border.

Original languageEnglish (US)
Pages (from-to)2193-2199
Number of pages7
JournalGenes and Development
Volume9
Issue number17
StatePublished - 1995
Externally publishedYes

Fingerprint

V(D)J Recombination
Genetic Recombination
Protein Sorting Signals
Mutation
DNA

Keywords

  • mutagenesis
  • plasmid library
  • RAG-1
  • V(D)J recombination

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

RAG-1 mutations that affect the target specificity of V(D)J recombination : A possible direct role of RAG-1 in site recognition. / Sadofsky, Moshe J.; Hesse, J. E.; Van Gent, D. C.; Gellert, M.

In: Genes and Development, Vol. 9, No. 17, 1995, p. 2193-2199.

Research output: Contribution to journalArticle

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