Radiosensitization of Murine Ehrlich ascites tumor by a combination of 2-deoxy-D-glucose and 6-aminonicotinamide

R. Varshney, S. Gupta, B. S. Dwarakanath

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Enhanced radiosensitizing effects of a combination of 2-deoxy-D-glucose (2-DG), a glycolytic inhibitor and 6-aminonicotinamide (6-AN) an analogue of nicotinamide, which inhibits hexose monophosphate shunt (HMP) have been demonstrated in vitro. The purpose of the present studies is to investigate in vivo effects of this combination in Ehrlich ascites tumor (EAT) bearing mice. EAT tumor was grown in Swiss albino strain A mice. Treatment induced growth delay and tumor free animal survival were evaluated as parameters of radiation response. Focal irradiation of the tumor with a single fraction of 10 Gy induced a moderate delay in tumor growth but did not lead to complete regression of the tumor. Intravenous administration of either 6-AN or 2-DG immediately before irradiation enhanced radiation-induced growth delay with a cure rate of 45%. However, administration of a combination of 2-DG (2 g/kg b.wt.) and 6-AN (2 mg/kg b.wt.) immediately before irradiation led to complete regression of tumor in 80% animals resulting in survival of more than 300 days. A similar response (≈80%) was observed when 2-DG dose was reduced to 1 g/kg in combination with 6-AN. It is concluded that 6-AN enhances the radiosensitizing effects of 2-DG and the combination may have potential application in improving radiotherapy of tumors.

Original languageEnglish (US)
Pages (from-to)659-663
Number of pages5
JournalTechnology in Cancer Research and Treatment
Volume3
Issue number6
DOIs
StatePublished - Dec 2004

Keywords

  • 2-deoxy-D-Glucose (2-DG)
  • 6-aminonicotinamide (6-AN)
  • Cure rate
  • Ehrlich Ascites Tumor (EAT)

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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