Radiolabeling antibodies with holmium-166

Ekaterina Dadachova, Saed Mirzadeh, Suzanne V. Smith, F. F. Knapp, Eric L. Hetherington

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Abstract

We report the preliminary results from radiolabeling of a chelate-conjugated antibody with 166Ho produced from the β--decay of 166Dy. Ho-166 was separated from mg quantities of Dy target by reverse phase ion-exchange chromatography employing a cation exchange HPLC column and 0.085 M α-HIBA at pH = 4.3 as eluent. Evaporation to dryness of 166Ho fraction (upto 25 mL) and thermal decomposition of α-HIBA yielded 166Ho in a dry state which was then solubilized in 0.5 mL of 0.1 M HCl. Subsequent radiolabeling of CHX-B-DTPA conjugated 135-14 monoclonal antibodies with purifled 166Ho was readily achieved with ~80% efficiency and with a specific activity of 3-4 mCi of 166Ho per mg of protein. 166Ho-antibody conjugates are stable with regards to transferrin challenge for a period of 50 h. Further, it was shown that any Fe3+ ions present in α-HIBA as an impurity interfere with the labeling.

Original languageEnglish (US)
Pages (from-to)477-481
Number of pages5
JournalApplied Radiation and Isotopes
Volume48
Issue number4
DOIs
StatePublished - Apr 1997

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ASJC Scopus subject areas

  • Radiation

Cite this

Dadachova, E., Mirzadeh, S., Smith, S. V., Knapp, F. F., & Hetherington, E. L. (1997). Radiolabeling antibodies with holmium-166. Applied Radiation and Isotopes, 48(4), 477-481. https://doi.org/10.1016/S0969-8043(96)00269-2