Radiolabeling antibodies with holmium-166

Ekaterina Dadachova, Saed Mirzadeh, Suzanne V. Smith, F. F. Knapp, Eric L. Hetherington

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We report the preliminary results from radiolabeling of a chelate-conjugated antibody with 166Ho produced from the β--decay of 166Dy. Ho-166 was separated from mg quantities of Dy target by reverse phase ion-exchange chromatography employing a cation exchange HPLC column and 0.085 M α-HIBA at pH = 4.3 as eluent. Evaporation to dryness of 166Ho fraction (upto 25 mL) and thermal decomposition of α-HIBA yielded 166Ho in a dry state which was then solubilized in 0.5 mL of 0.1 M HCl. Subsequent radiolabeling of CHX-B-DTPA conjugated 135-14 monoclonal antibodies with purifled 166Ho was readily achieved with ~80% efficiency and with a specific activity of 3-4 mCi of 166Ho per mg of protein. 166Ho-antibody conjugates are stable with regards to transferrin challenge for a period of 50 h. Further, it was shown that any Fe3+ ions present in α-HIBA as an impurity interfere with the labeling.

Original languageEnglish (US)
Pages (from-to)477-481
Number of pages5
JournalApplied Radiation and Isotopes
Volume48
Issue number4
DOIs
StatePublished - 1997
Externally publishedYes

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holmium
antibodies
chromatography
chelates
marking
thermal decomposition
ions
evaporation
proteins
cations
impurities
decay

ASJC Scopus subject areas

  • Radiation

Cite this

Dadachova, E., Mirzadeh, S., Smith, S. V., Knapp, F. F., & Hetherington, E. L. (1997). Radiolabeling antibodies with holmium-166. Applied Radiation and Isotopes, 48(4), 477-481. https://doi.org/10.1016/S0969-8043(96)00269-2

Radiolabeling antibodies with holmium-166. / Dadachova, Ekaterina; Mirzadeh, Saed; Smith, Suzanne V.; Knapp, F. F.; Hetherington, Eric L.

In: Applied Radiation and Isotopes, Vol. 48, No. 4, 1997, p. 477-481.

Research output: Contribution to journalArticle

Dadachova, E, Mirzadeh, S, Smith, SV, Knapp, FF & Hetherington, EL 1997, 'Radiolabeling antibodies with holmium-166', Applied Radiation and Isotopes, vol. 48, no. 4, pp. 477-481. https://doi.org/10.1016/S0969-8043(96)00269-2
Dadachova E, Mirzadeh S, Smith SV, Knapp FF, Hetherington EL. Radiolabeling antibodies with holmium-166. Applied Radiation and Isotopes. 1997;48(4):477-481. https://doi.org/10.1016/S0969-8043(96)00269-2
Dadachova, Ekaterina ; Mirzadeh, Saed ; Smith, Suzanne V. ; Knapp, F. F. ; Hetherington, Eric L. / Radiolabeling antibodies with holmium-166. In: Applied Radiation and Isotopes. 1997 ; Vol. 48, No. 4. pp. 477-481.
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