Radioimmunotherapy of Cryptococcus neoformans spares bystander mammalian cells

Ruth A. Bryan, Zewei Jiang, Alfred Morgenstern, Frank Bruchertseifer, Arturo Casadevall, Ekaterina Dadachova

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Aim: Previously, we showed that radioimmunotherapy (RIT) for cryptococcal infections using radioactively labeled antibodies recognizing the cryptococcal capsule reduced fungal burden and prolonged survival of mice infected with Cryptococcus neoformans. Here, we investigate the effects of RIT on bystander mammalian cells. Materials & methods: Heat-killed C. neoformans bound to anticapsular antibodies, unlabeled or labeled with the α-emitter rhenium-188 (16.9-h half-life) or the α-emitter bismuth-213 (46-min half-life), was incubated with macrophage-like J774.16 cells or epithelial-like Chinese hamster ovary cells. Lactate dehydrogenase activity, crystal violet uptake, reduction of tetrazolium dye (2,3)-bis-(2-methoxy-4-nitro-5-sulfenyl)- (2H)-terazolium-5-carboxanilide and nitric oxide production were measured. Results: The J774.16 and Chinese hamster ovary cells maintained membrane integrity, viability and metabolic activity following exposure to radiolabeled C. neoformans.Conclusion: RIT of C. neoformans is a selective therapy with minimal effects on host cells and these results are consistent with observations that RIT-treated mice with cryptococcal infection lacked RIT-related pathological changes in lungs and brain tissues.

Original languageEnglish (US)
Pages (from-to)1081-1089
Number of pages9
JournalFuture Microbiology
Volume8
Issue number9
DOIs
StatePublished - Sep 2013

Fingerprint

Radioimmunotherapy
Cryptococcus neoformans
Cricetulus
Fungal Capsules
Half-Life
Ovary
Rhenium
Gentian Violet
Bismuth
Antibodies
Infection
L-Lactate Dehydrogenase
Nitric Oxide
Hot Temperature
Epithelial Cells
Macrophages
Cell Membrane
Lung
Brain

Keywords

  • bystander effects
  • Cryptococcus neoformans
  • fungal infection
  • NO production
  • particulate radiation
  • radioimmunotherapy

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Bryan, R. A., Jiang, Z., Morgenstern, A., Bruchertseifer, F., Casadevall, A., & Dadachova, E. (2013). Radioimmunotherapy of Cryptococcus neoformans spares bystander mammalian cells. Future Microbiology, 8(9), 1081-1089. https://doi.org/10.2217/fmb.13.79

Radioimmunotherapy of Cryptococcus neoformans spares bystander mammalian cells. / Bryan, Ruth A.; Jiang, Zewei; Morgenstern, Alfred; Bruchertseifer, Frank; Casadevall, Arturo; Dadachova, Ekaterina.

In: Future Microbiology, Vol. 8, No. 9, 09.2013, p. 1081-1089.

Research output: Contribution to journalArticle

Bryan, RA, Jiang, Z, Morgenstern, A, Bruchertseifer, F, Casadevall, A & Dadachova, E 2013, 'Radioimmunotherapy of Cryptococcus neoformans spares bystander mammalian cells', Future Microbiology, vol. 8, no. 9, pp. 1081-1089. https://doi.org/10.2217/fmb.13.79
Bryan RA, Jiang Z, Morgenstern A, Bruchertseifer F, Casadevall A, Dadachova E. Radioimmunotherapy of Cryptococcus neoformans spares bystander mammalian cells. Future Microbiology. 2013 Sep;8(9):1081-1089. https://doi.org/10.2217/fmb.13.79
Bryan, Ruth A. ; Jiang, Zewei ; Morgenstern, Alfred ; Bruchertseifer, Frank ; Casadevall, Arturo ; Dadachova, Ekaterina. / Radioimmunotherapy of Cryptococcus neoformans spares bystander mammalian cells. In: Future Microbiology. 2013 ; Vol. 8, No. 9. pp. 1081-1089.
@article{93bd740328344f29ac1d57d3a369318b,
title = "Radioimmunotherapy of Cryptococcus neoformans spares bystander mammalian cells",
abstract = "Aim: Previously, we showed that radioimmunotherapy (RIT) for cryptococcal infections using radioactively labeled antibodies recognizing the cryptococcal capsule reduced fungal burden and prolonged survival of mice infected with Cryptococcus neoformans. Here, we investigate the effects of RIT on bystander mammalian cells. Materials & methods: Heat-killed C. neoformans bound to anticapsular antibodies, unlabeled or labeled with the α-emitter rhenium-188 (16.9-h half-life) or the α-emitter bismuth-213 (46-min half-life), was incubated with macrophage-like J774.16 cells or epithelial-like Chinese hamster ovary cells. Lactate dehydrogenase activity, crystal violet uptake, reduction of tetrazolium dye (2,3)-bis-(2-methoxy-4-nitro-5-sulfenyl)- (2H)-terazolium-5-carboxanilide and nitric oxide production were measured. Results: The J774.16 and Chinese hamster ovary cells maintained membrane integrity, viability and metabolic activity following exposure to radiolabeled C. neoformans.Conclusion: RIT of C. neoformans is a selective therapy with minimal effects on host cells and these results are consistent with observations that RIT-treated mice with cryptococcal infection lacked RIT-related pathological changes in lungs and brain tissues.",
keywords = "bystander effects, Cryptococcus neoformans, fungal infection, NO production, particulate radiation, radioimmunotherapy",
author = "Bryan, {Ruth A.} and Zewei Jiang and Alfred Morgenstern and Frank Bruchertseifer and Arturo Casadevall and Ekaterina Dadachova",
year = "2013",
month = "9",
doi = "10.2217/fmb.13.79",
language = "English (US)",
volume = "8",
pages = "1081--1089",
journal = "Future Microbiology",
issn = "1746-0913",
publisher = "Future Medicine Ltd.",
number = "9",

}

TY - JOUR

T1 - Radioimmunotherapy of Cryptococcus neoformans spares bystander mammalian cells

AU - Bryan, Ruth A.

AU - Jiang, Zewei

AU - Morgenstern, Alfred

AU - Bruchertseifer, Frank

AU - Casadevall, Arturo

AU - Dadachova, Ekaterina

PY - 2013/9

Y1 - 2013/9

N2 - Aim: Previously, we showed that radioimmunotherapy (RIT) for cryptococcal infections using radioactively labeled antibodies recognizing the cryptococcal capsule reduced fungal burden and prolonged survival of mice infected with Cryptococcus neoformans. Here, we investigate the effects of RIT on bystander mammalian cells. Materials & methods: Heat-killed C. neoformans bound to anticapsular antibodies, unlabeled or labeled with the α-emitter rhenium-188 (16.9-h half-life) or the α-emitter bismuth-213 (46-min half-life), was incubated with macrophage-like J774.16 cells or epithelial-like Chinese hamster ovary cells. Lactate dehydrogenase activity, crystal violet uptake, reduction of tetrazolium dye (2,3)-bis-(2-methoxy-4-nitro-5-sulfenyl)- (2H)-terazolium-5-carboxanilide and nitric oxide production were measured. Results: The J774.16 and Chinese hamster ovary cells maintained membrane integrity, viability and metabolic activity following exposure to radiolabeled C. neoformans.Conclusion: RIT of C. neoformans is a selective therapy with minimal effects on host cells and these results are consistent with observations that RIT-treated mice with cryptococcal infection lacked RIT-related pathological changes in lungs and brain tissues.

AB - Aim: Previously, we showed that radioimmunotherapy (RIT) for cryptococcal infections using radioactively labeled antibodies recognizing the cryptococcal capsule reduced fungal burden and prolonged survival of mice infected with Cryptococcus neoformans. Here, we investigate the effects of RIT on bystander mammalian cells. Materials & methods: Heat-killed C. neoformans bound to anticapsular antibodies, unlabeled or labeled with the α-emitter rhenium-188 (16.9-h half-life) or the α-emitter bismuth-213 (46-min half-life), was incubated with macrophage-like J774.16 cells or epithelial-like Chinese hamster ovary cells. Lactate dehydrogenase activity, crystal violet uptake, reduction of tetrazolium dye (2,3)-bis-(2-methoxy-4-nitro-5-sulfenyl)- (2H)-terazolium-5-carboxanilide and nitric oxide production were measured. Results: The J774.16 and Chinese hamster ovary cells maintained membrane integrity, viability and metabolic activity following exposure to radiolabeled C. neoformans.Conclusion: RIT of C. neoformans is a selective therapy with minimal effects on host cells and these results are consistent with observations that RIT-treated mice with cryptococcal infection lacked RIT-related pathological changes in lungs and brain tissues.

KW - bystander effects

KW - Cryptococcus neoformans

KW - fungal infection

KW - NO production

KW - particulate radiation

KW - radioimmunotherapy

UR - http://www.scopus.com/inward/record.url?scp=84884124932&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884124932&partnerID=8YFLogxK

U2 - 10.2217/fmb.13.79

DO - 10.2217/fmb.13.79

M3 - Article

VL - 8

SP - 1081

EP - 1089

JO - Future Microbiology

JF - Future Microbiology

SN - 1746-0913

IS - 9

ER -