Radioimmunoassay for the measurement of mass and avidity of anti-nerve growth factor antibodies

Roberto Revoltella, Ruth Hogue Angeletti, Marcella Pediconi, Luisa Bertolini

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Abstract

A radioimmunoassay is described for measuring on a weight basis the total amount of humoral antibodies to the purified protein nerve growth factor (NGF). The technique is based on the primary interaction of the antibodies with 125I-labelled NGF added in large molar excess. The resultant immune complexes are precipitated by ammonium sulfate at 37% saturation, while unbound 125I-labelled NGF is removed with the supernatant. The assay permits measurement of antibodies in a range of sensitivity of 0.1-1.0 ng antibody protein per ml. It is specific and reproducible and permits estimates of the antibody avidity.

Original languageEnglish (US)
Pages (from-to)67-82
Number of pages16
JournalJournal of Immunological Methods
Volume4
Issue number1
DOIs
Publication statusPublished - Jan 1974

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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