Radioimmunoassay for the measurement of mass and avidity of anti-nerve growth factor antibodies

Roberto Revoltella, Ruth Hogue Angeletti, Marcella Pediconi, Luisa Bertolini

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A radioimmunoassay is described for measuring on a weight basis the total amount of humoral antibodies to the purified protein nerve growth factor (NGF). The technique is based on the primary interaction of the antibodies with 125I-labelled NGF added in large molar excess. The resultant immune complexes are precipitated by ammonium sulfate at 37% saturation, while unbound 125I-labelled NGF is removed with the supernatant. The assay permits measurement of antibodies in a range of sensitivity of 0.1-1.0 ng antibody protein per ml. It is specific and reproducible and permits estimates of the antibody avidity.

Original languageEnglish (US)
Pages (from-to)67-82
Number of pages16
JournalJournal of Immunological Methods
Volume4
Issue number1
DOIs
StatePublished - 1974
Externally publishedYes

Fingerprint

Nerve Growth Factor
Radioimmunoassay
Antibodies
Antibody Affinity
Ammonium Sulfate
Antigen-Antibody Complex
Proteins
Weights and Measures

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Radioimmunoassay for the measurement of mass and avidity of anti-nerve growth factor antibodies. / Revoltella, Roberto; Angeletti, Ruth Hogue; Pediconi, Marcella; Bertolini, Luisa.

In: Journal of Immunological Methods, Vol. 4, No. 1, 1974, p. 67-82.

Research output: Contribution to journalArticle

Revoltella, Roberto ; Angeletti, Ruth Hogue ; Pediconi, Marcella ; Bertolini, Luisa. / Radioimmunoassay for the measurement of mass and avidity of anti-nerve growth factor antibodies. In: Journal of Immunological Methods. 1974 ; Vol. 4, No. 1. pp. 67-82.
@article{d4f8d2ce6e0e40b4bbab6f38566a8f7c,
title = "Radioimmunoassay for the measurement of mass and avidity of anti-nerve growth factor antibodies",
abstract = "A radioimmunoassay is described for measuring on a weight basis the total amount of humoral antibodies to the purified protein nerve growth factor (NGF). The technique is based on the primary interaction of the antibodies with 125I-labelled NGF added in large molar excess. The resultant immune complexes are precipitated by ammonium sulfate at 37{\%} saturation, while unbound 125I-labelled NGF is removed with the supernatant. The assay permits measurement of antibodies in a range of sensitivity of 0.1-1.0 ng antibody protein per ml. It is specific and reproducible and permits estimates of the antibody avidity.",
author = "Roberto Revoltella and Angeletti, {Ruth Hogue} and Marcella Pediconi and Luisa Bertolini",
year = "1974",
doi = "10.1016/0022-1759(74)90035-0",
language = "English (US)",
volume = "4",
pages = "67--82",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Radioimmunoassay for the measurement of mass and avidity of anti-nerve growth factor antibodies

AU - Revoltella, Roberto

AU - Angeletti, Ruth Hogue

AU - Pediconi, Marcella

AU - Bertolini, Luisa

PY - 1974

Y1 - 1974

N2 - A radioimmunoassay is described for measuring on a weight basis the total amount of humoral antibodies to the purified protein nerve growth factor (NGF). The technique is based on the primary interaction of the antibodies with 125I-labelled NGF added in large molar excess. The resultant immune complexes are precipitated by ammonium sulfate at 37% saturation, while unbound 125I-labelled NGF is removed with the supernatant. The assay permits measurement of antibodies in a range of sensitivity of 0.1-1.0 ng antibody protein per ml. It is specific and reproducible and permits estimates of the antibody avidity.

AB - A radioimmunoassay is described for measuring on a weight basis the total amount of humoral antibodies to the purified protein nerve growth factor (NGF). The technique is based on the primary interaction of the antibodies with 125I-labelled NGF added in large molar excess. The resultant immune complexes are precipitated by ammonium sulfate at 37% saturation, while unbound 125I-labelled NGF is removed with the supernatant. The assay permits measurement of antibodies in a range of sensitivity of 0.1-1.0 ng antibody protein per ml. It is specific and reproducible and permits estimates of the antibody avidity.

UR - http://www.scopus.com/inward/record.url?scp=0015955563&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015955563&partnerID=8YFLogxK

U2 - 10.1016/0022-1759(74)90035-0

DO - 10.1016/0022-1759(74)90035-0

M3 - Article

VL - 4

SP - 67

EP - 82

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1

ER -