Radiation dose-dependent changes in tumor metabolism measured by 31P nuclear magnetic resonance spectroscopy

Umar Mahmood, Alan A. Alfieri, Howard Thaler, David Cowburn, Jason A. Koutcher

Research output: Contribution to journalArticle

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Abstract

The effects of radiation dose upon a hypoxic murine mammary carcinoma were followed using 31P nuclear magnetic resonance spectroscopy. Animals were studied before and over the course of 9 days after tumors were irradiated with a single dose of 0, 4, 8, or 17 Gy. The current data is compared to our previous studies of the effects of 32 or 65 Gy on the same tumor model. The energy status of the tumors, as reflected in nucleotide triphosphate:Pi and phosphocreatine:Pi ratios, improved after receiving a dose of 8 to 65 Gy and decreased after receiving 0 or 4 Gy doses. The energy status of the 8- to 65-Gy dose cohorts reached a maximum between 1 and 4 days after irradiation. Additionally, the change in the hypoxic cell fraction 48 h after a 17-Gy dose was determined; it was calculated from changes in the doses required to control 50% of the tumors post radiation for clamped (hypoxic) and unclamped (normoxic) tumors in parallel animal cohorts. A significant decrease compared to preirradiation values was observed in the hypoxic cell fraction following 17 Gy irradiation. This decrease was temporally coincident with increases in tumor energy status measured using nuclear magnetic resonance and was similar to our previously reported results of the change in hypoxic fraction 48 h after a 32-Gy dose. Changes in the relative ratio of phosphomonoesters showed a strong dose dependence after irradiation. The downfield component of the phosphomonoester peak, which consists largely of phosphoethanolamine, increased relative to the upfield component, phosphocholine. This dosedependent ratio reached a maximum approximately 7 days post radiation. Changes in the levels of membrane phospholipid precursors may be related to alterations in cell proliferation or may be a result of radiation-induced membrane damage.

Original languageEnglish (US)
Pages (from-to)4885-4891
Number of pages7
JournalCancer Research
Volume54
Issue number18
StatePublished - Sep 15 1994
Externally publishedYes

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Magnetic Resonance Spectroscopy
Radiation
Neoplasms
Phosphorylcholine
Membranes
Phosphocreatine
Radiation Effects
Phospholipids
Nucleotides
Cell Proliferation
Breast Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Radiation dose-dependent changes in tumor metabolism measured by 31P nuclear magnetic resonance spectroscopy. / Mahmood, Umar; Alfieri, Alan A.; Thaler, Howard; Cowburn, David; Koutcher, Jason A.

In: Cancer Research, Vol. 54, No. 18, 15.09.1994, p. 4885-4891.

Research output: Contribution to journalArticle

Mahmood, U, Alfieri, AA, Thaler, H, Cowburn, D & Koutcher, JA 1994, 'Radiation dose-dependent changes in tumor metabolism measured by 31P nuclear magnetic resonance spectroscopy', Cancer Research, vol. 54, no. 18, pp. 4885-4891.
Mahmood, Umar ; Alfieri, Alan A. ; Thaler, Howard ; Cowburn, David ; Koutcher, Jason A. / Radiation dose-dependent changes in tumor metabolism measured by 31P nuclear magnetic resonance spectroscopy. In: Cancer Research. 1994 ; Vol. 54, No. 18. pp. 4885-4891.
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