TY - JOUR
T1 - Racial differences in selected cytokine allelic and genotypic frequencies among healthy, pregnant women in North Carolina
AU - Hassan, Mohamed I.
AU - Aschner, Yael
AU - Manning, Carolina H.
AU - Xu, Jianfeng
AU - Aschner, Judy L.
N1 - Funding Information:
This study was supported by a grant from ‘A Grant Program for Fellows in Neonatology’ from Forest Pharmaceuticals, and by the WFUSM General Clinical Research Center (Grant # M01-RR07122). We gratefully acknowledge the technical assistance of the personnel in the Histocompatibility Laboratory at Wake Forest University School of Medicine.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2003/1/7
Y1 - 2003/1/7
N2 - Background: Genetic susceptibility to diseases is likely influenced by common DNA variants in the form of single nucleotide polymorphisms (SNPs). The value of SNPs for linkage and association mapping studies may depend on the distribution of SNP allele frequencies across populations. Objectives: To establish the SNP allelic frequencies among Caucasian and African American women for tumor necrosis factor (TNF)α, transforming growth factor (TGF)β1, interleukin-10 (IL10), interleukin-6 (IL6), and interferon (IFN)γ. Materials and methods: DNA was extracted from whole blood from 123 healthy, pregnant women. PCR-based genotyping was performed for the genes encoding TNFα (-308G/A), TGFβ1 (codon 10C/T, codon 25C/G), IL10 (-1082A/G, -819T/C, -592A/C), IL6 (-174C/G) and IFNγ (874T/A). Allele frequencies were determined by Hardy-Weinberg Equilibrium and Linkage Disequilibrium tests. Differences in the SNP allelic frequencies between Caucasians and African Americans were assessed by the χ2 of Amitage trend test. Results: SNP allelic and genotypic frequencies for IL6 and IFNγ, but not for TNFα, TGFβ1, and IL10, differed significantly between the Caucasian and African American women. Conclusions: Recognition of racial differences in SNP allelic and genotypic frequencies for selected cytokines is important for designing and powering future linkage and association mapping studies investigating the role of cytokines in human disease.
AB - Background: Genetic susceptibility to diseases is likely influenced by common DNA variants in the form of single nucleotide polymorphisms (SNPs). The value of SNPs for linkage and association mapping studies may depend on the distribution of SNP allele frequencies across populations. Objectives: To establish the SNP allelic frequencies among Caucasian and African American women for tumor necrosis factor (TNF)α, transforming growth factor (TGF)β1, interleukin-10 (IL10), interleukin-6 (IL6), and interferon (IFN)γ. Materials and methods: DNA was extracted from whole blood from 123 healthy, pregnant women. PCR-based genotyping was performed for the genes encoding TNFα (-308G/A), TGFβ1 (codon 10C/T, codon 25C/G), IL10 (-1082A/G, -819T/C, -592A/C), IL6 (-174C/G) and IFNγ (874T/A). Allele frequencies were determined by Hardy-Weinberg Equilibrium and Linkage Disequilibrium tests. Differences in the SNP allelic frequencies between Caucasians and African Americans were assessed by the χ2 of Amitage trend test. Results: SNP allelic and genotypic frequencies for IL6 and IFNγ, but not for TNFα, TGFβ1, and IL10, differed significantly between the Caucasian and African American women. Conclusions: Recognition of racial differences in SNP allelic and genotypic frequencies for selected cytokines is important for designing and powering future linkage and association mapping studies investigating the role of cytokines in human disease.
KW - Cytokines
KW - Ethnicity
KW - Single nucleotide polymorphisms
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U2 - 10.1016/S1043-4666(02)00489-1
DO - 10.1016/S1043-4666(02)00489-1
M3 - Article
C2 - 12668154
AN - SCOPUS:0037422705
SN - 1043-4666
VL - 21
SP - 10
EP - 16
JO - Cytokine
JF - Cytokine
IS - 1
ER -