Racial differences in selected cytokine allelic and genotypic frequencies among healthy, pregnant women in North Carolina

Mohamed I. Hassan, Yael Aschner, Carolina H. Manning, Jianfeng Xu, Judy L. Aschner

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Background: Genetic susceptibility to diseases is likely influenced by common DNA variants in the form of single nucleotide polymorphisms (SNPs). The value of SNPs for linkage and association mapping studies may depend on the distribution of SNP allele frequencies across populations. Objectives: To establish the SNP allelic frequencies among Caucasian and African American women for tumor necrosis factor (TNF)α, transforming growth factor (TGF)β1, interleukin-10 (IL10), interleukin-6 (IL6), and interferon (IFN)γ. Materials and methods: DNA was extracted from whole blood from 123 healthy, pregnant women. PCR-based genotyping was performed for the genes encoding TNFα (-308G/A), TGFβ1 (codon 10C/T, codon 25C/G), IL10 (-1082A/G, -819T/C, -592A/C), IL6 (-174C/G) and IFNγ (874T/A). Allele frequencies were determined by Hardy-Weinberg Equilibrium and Linkage Disequilibrium tests. Differences in the SNP allelic frequencies between Caucasians and African Americans were assessed by the χ2 of Amitage trend test. Results: SNP allelic and genotypic frequencies for IL6 and IFNγ, but not for TNFα, TGFβ1, and IL10, differed significantly between the Caucasian and African American women. Conclusions: Recognition of racial differences in SNP allelic and genotypic frequencies for selected cytokines is important for designing and powering future linkage and association mapping studies investigating the role of cytokines in human disease.

Original languageEnglish (US)
Pages (from-to)10-16
Number of pages7
JournalCytokine
Volume21
Issue number1
DOIs
StatePublished - Jan 7 2003
Externally publishedYes

Fingerprint

Polymorphism
Single Nucleotide Polymorphism
Pregnant Women
Nucleotides
Cytokines
African Americans
Interleukin-10
Interferons
Interleukin-6
Tumor Necrosis Factor-alpha
Chromosome Mapping
Gene Frequency
Codon
Gene encoding
DNA
Linkage Disequilibrium
Transforming Growth Factors
Genetic Predisposition to Disease
Blood
Polymerase Chain Reaction

Keywords

  • Cytokines
  • Ethnicity
  • Single nucleotide polymorphisms

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Racial differences in selected cytokine allelic and genotypic frequencies among healthy, pregnant women in North Carolina. / Hassan, Mohamed I.; Aschner, Yael; Manning, Carolina H.; Xu, Jianfeng; Aschner, Judy L.

In: Cytokine, Vol. 21, No. 1, 07.01.2003, p. 10-16.

Research output: Contribution to journalArticle

Hassan, Mohamed I. ; Aschner, Yael ; Manning, Carolina H. ; Xu, Jianfeng ; Aschner, Judy L. / Racial differences in selected cytokine allelic and genotypic frequencies among healthy, pregnant women in North Carolina. In: Cytokine. 2003 ; Vol. 21, No. 1. pp. 10-16.
@article{23a89cbcdcb948669afc7e0f32b5527a,
title = "Racial differences in selected cytokine allelic and genotypic frequencies among healthy, pregnant women in North Carolina",
abstract = "Background: Genetic susceptibility to diseases is likely influenced by common DNA variants in the form of single nucleotide polymorphisms (SNPs). The value of SNPs for linkage and association mapping studies may depend on the distribution of SNP allele frequencies across populations. Objectives: To establish the SNP allelic frequencies among Caucasian and African American women for tumor necrosis factor (TNF)α, transforming growth factor (TGF)β1, interleukin-10 (IL10), interleukin-6 (IL6), and interferon (IFN)γ. Materials and methods: DNA was extracted from whole blood from 123 healthy, pregnant women. PCR-based genotyping was performed for the genes encoding TNFα (-308G/A), TGFβ1 (codon 10C/T, codon 25C/G), IL10 (-1082A/G, -819T/C, -592A/C), IL6 (-174C/G) and IFNγ (874T/A). Allele frequencies were determined by Hardy-Weinberg Equilibrium and Linkage Disequilibrium tests. Differences in the SNP allelic frequencies between Caucasians and African Americans were assessed by the χ2 of Amitage trend test. Results: SNP allelic and genotypic frequencies for IL6 and IFNγ, but not for TNFα, TGFβ1, and IL10, differed significantly between the Caucasian and African American women. Conclusions: Recognition of racial differences in SNP allelic and genotypic frequencies for selected cytokines is important for designing and powering future linkage and association mapping studies investigating the role of cytokines in human disease.",
keywords = "Cytokines, Ethnicity, Single nucleotide polymorphisms",
author = "Hassan, {Mohamed I.} and Yael Aschner and Manning, {Carolina H.} and Jianfeng Xu and Aschner, {Judy L.}",
year = "2003",
month = "1",
day = "7",
doi = "10.1016/S1043-4666(02)00489-1",
language = "English (US)",
volume = "21",
pages = "10--16",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Racial differences in selected cytokine allelic and genotypic frequencies among healthy, pregnant women in North Carolina

AU - Hassan, Mohamed I.

AU - Aschner, Yael

AU - Manning, Carolina H.

AU - Xu, Jianfeng

AU - Aschner, Judy L.

PY - 2003/1/7

Y1 - 2003/1/7

N2 - Background: Genetic susceptibility to diseases is likely influenced by common DNA variants in the form of single nucleotide polymorphisms (SNPs). The value of SNPs for linkage and association mapping studies may depend on the distribution of SNP allele frequencies across populations. Objectives: To establish the SNP allelic frequencies among Caucasian and African American women for tumor necrosis factor (TNF)α, transforming growth factor (TGF)β1, interleukin-10 (IL10), interleukin-6 (IL6), and interferon (IFN)γ. Materials and methods: DNA was extracted from whole blood from 123 healthy, pregnant women. PCR-based genotyping was performed for the genes encoding TNFα (-308G/A), TGFβ1 (codon 10C/T, codon 25C/G), IL10 (-1082A/G, -819T/C, -592A/C), IL6 (-174C/G) and IFNγ (874T/A). Allele frequencies were determined by Hardy-Weinberg Equilibrium and Linkage Disequilibrium tests. Differences in the SNP allelic frequencies between Caucasians and African Americans were assessed by the χ2 of Amitage trend test. Results: SNP allelic and genotypic frequencies for IL6 and IFNγ, but not for TNFα, TGFβ1, and IL10, differed significantly between the Caucasian and African American women. Conclusions: Recognition of racial differences in SNP allelic and genotypic frequencies for selected cytokines is important for designing and powering future linkage and association mapping studies investigating the role of cytokines in human disease.

AB - Background: Genetic susceptibility to diseases is likely influenced by common DNA variants in the form of single nucleotide polymorphisms (SNPs). The value of SNPs for linkage and association mapping studies may depend on the distribution of SNP allele frequencies across populations. Objectives: To establish the SNP allelic frequencies among Caucasian and African American women for tumor necrosis factor (TNF)α, transforming growth factor (TGF)β1, interleukin-10 (IL10), interleukin-6 (IL6), and interferon (IFN)γ. Materials and methods: DNA was extracted from whole blood from 123 healthy, pregnant women. PCR-based genotyping was performed for the genes encoding TNFα (-308G/A), TGFβ1 (codon 10C/T, codon 25C/G), IL10 (-1082A/G, -819T/C, -592A/C), IL6 (-174C/G) and IFNγ (874T/A). Allele frequencies were determined by Hardy-Weinberg Equilibrium and Linkage Disequilibrium tests. Differences in the SNP allelic frequencies between Caucasians and African Americans were assessed by the χ2 of Amitage trend test. Results: SNP allelic and genotypic frequencies for IL6 and IFNγ, but not for TNFα, TGFβ1, and IL10, differed significantly between the Caucasian and African American women. Conclusions: Recognition of racial differences in SNP allelic and genotypic frequencies for selected cytokines is important for designing and powering future linkage and association mapping studies investigating the role of cytokines in human disease.

KW - Cytokines

KW - Ethnicity

KW - Single nucleotide polymorphisms

UR - http://www.scopus.com/inward/record.url?scp=0037422705&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037422705&partnerID=8YFLogxK

U2 - 10.1016/S1043-4666(02)00489-1

DO - 10.1016/S1043-4666(02)00489-1

M3 - Article

VL - 21

SP - 10

EP - 16

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 1

ER -