The power-dependent behavior of the resonance Raman spectra of deoxy hemoglobin was studied by using ˜30-psec laser pulses. The linewidth of a Raman active mode that is sensitive to porphyrin π-electron density, V4, broadens asymmetrically as a function of laser fluence. Several possible origins of this phenomenon are examined, including the presence of a transient photoproduct and a nonlinear change in the resonance Raman scattering process through Rabi broadening. Our data are consistent with the second possibility and can be qualitatively modeled in simulations that se realistic values of T1 and T2 for the heme Soret electronic transition.
|Original language||English (US)|
|Number of pages||7|
|Journal||Journal of the Optical Society of America B: Optical Physics|
|Publication status||Published - 1990|
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Statistical and Nonlinear Physics