Rab6c, a new member of the Rab gene family, is involved in drug resistance in MCF7/AdrR cells

Jidong Shan, James M. Mason, Liming Yuan, Michele Barcia, Debra Porti, Anthony Calabro, Daniel Budman, Vincent Vinciguerra, Hao Peng Xu

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

A new Rab6 homolog cDNA, Rab6c, was discovered by a hypermethylated DNA fragment probe that was isolated from a human multidrug resistant (MDR) breast cancer cell line, MCF7/AdrR, by the methylation sensitive-representational difference analysis (MS-RDA) technique. Rab6c was found to be under-expressed in MCF7/AdrR and MES-SA/Dx5 (a human MDR uterine sarcoma cell line) compared with their non-MDR parental cell lines. MCF7/AdrR cells expressing the exogenous Rab6c exhibited less resistance to several anti-cancer drugs, such as doxorubicin (DOX), taxol, vinblastine, and vincristine, than the control cells containing the empty vector. Flow cytometry experiments confirmed that the transfectants' diminished resistance to DOX was caused by increased drug accumulation induced by the exogenous Rab6c. These results indicate that Rab6c is involved in drug resistance in MCF7/AdrR cells. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)67-75
Number of pages9
JournalGene
Volume257
Issue number1
DOIs
StatePublished - Oct 17 2000
Externally publishedYes

Keywords

  • G protein
  • Gene expression
  • Gene transfection
  • IC50
  • MS-RDA

ASJC Scopus subject areas

  • Genetics

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  • Cite this

    Shan, J., Mason, J. M., Yuan, L., Barcia, M., Porti, D., Calabro, A., Budman, D., Vinciguerra, V., & Xu, H. P. (2000). Rab6c, a new member of the Rab gene family, is involved in drug resistance in MCF7/AdrR cells. Gene, 257(1), 67-75. https://doi.org/10.1016/S0378-1119(00)00395-4