Rab5 regulates motility of early endosomes on microtubules

Erik Nielsen, Fedor Severin, Jonathan M. Backer, Anthony A. Hyman, Marino Zerial

Research output: Contribution to journalArticle

362 Citations (Scopus)

Abstract

The small GTPase Rab5 regulates membrane docking and fusion in the early endocytic pathway. Here we reveal a new role for Rab5 in the regulation of endosome interactions with the microtubule network. Using Rab5 fused to green fluorescent protein we show that Rab5-positive endosomes move on microtubules in vivo. In vitro, Rab5 stimulates both association of early endosomes with microtubules and early-endosome motility towards the minus ends of microtubules. Moreover, similarly to endosome membrane docking and fusion, Rab5-dependent endosome movement depends on the phosphatidylinositol-3-OH kinase hVPS34. Thus, Rab5 functionally links regulation of membrane transport, motility and intracellular distribution of early endosomes.

Original languageEnglish (US)
Pages (from-to)376-382
Number of pages7
JournalNature Cell Biology
Volume1
Issue number6
StatePublished - Oct 1999

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Endosomes
Microtubules
Membrane Fusion
Monomeric GTP-Binding Proteins
Green Fluorescent Proteins
Phosphatidylinositol 3-Kinases
Membranes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Nielsen, E., Severin, F., Backer, J. M., Hyman, A. A., & Zerial, M. (1999). Rab5 regulates motility of early endosomes on microtubules. Nature Cell Biology, 1(6), 376-382.

Rab5 regulates motility of early endosomes on microtubules. / Nielsen, Erik; Severin, Fedor; Backer, Jonathan M.; Hyman, Anthony A.; Zerial, Marino.

In: Nature Cell Biology, Vol. 1, No. 6, 10.1999, p. 376-382.

Research output: Contribution to journalArticle

Nielsen, E, Severin, F, Backer, JM, Hyman, AA & Zerial, M 1999, 'Rab5 regulates motility of early endosomes on microtubules', Nature Cell Biology, vol. 1, no. 6, pp. 376-382.
Nielsen E, Severin F, Backer JM, Hyman AA, Zerial M. Rab5 regulates motility of early endosomes on microtubules. Nature Cell Biology. 1999 Oct;1(6):376-382.
Nielsen, Erik ; Severin, Fedor ; Backer, Jonathan M. ; Hyman, Anthony A. ; Zerial, Marino. / Rab5 regulates motility of early endosomes on microtubules. In: Nature Cell Biology. 1999 ; Vol. 1, No. 6. pp. 376-382.
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