TY - JOUR
T1 - Quantitative rather than qualitative differences in gene expression predominate in intestinal cell maturation along distinct cell lineages
AU - Velcich, Anna
AU - Corner, Georgia
AU - Paul, Doru
AU - Zhuang, Min
AU - Mariadason, John M.
AU - Laboisse, Christian
AU - Augenlicht, Leonard
N1 - Funding Information:
This work was supported by grants CA90808, CA88104, and P013330 from the National Cancer Institute. We thank A. Massimi and J. Childs for generating and scanning the arrays; M. Houston and D. Arango for help in the cell cycle and apoptosis analysis.
PY - 2005/3/10
Y1 - 2005/3/10
N2 - Several cell types are present in the intestinal epithelium that likely arise from a common precursor, the stem cell, and each mature cell type expresses a unique set of genes that characterizes its functional phenotype. Although the process of differentiation is intimately linked to the cessation of proliferation, the mechanisms that dictate intestinal cell fate determination are not well characterized. To investigate the reprogramming of gene expression during the cell lineage allocation/differentiation process, we took advantage of a unique system of two clonal derivatives of HT29 cells, Cl16E and Cl19A cells, which spontaneously differentiate as mucus producing goblet and chloride-secreting cells, respectively, as a function of time. By profiling gene expression, we found that these two cell lines show remarkably similar kinetics of change in gene expression and common clusters of coordinately regulated genes. This demonstrates that lineage-specific differentiation of intestinal epithelial cells is characterized overall by the sequential recruitment of functionally similar gene sets independent of the final phenotype of the mature cells.
AB - Several cell types are present in the intestinal epithelium that likely arise from a common precursor, the stem cell, and each mature cell type expresses a unique set of genes that characterizes its functional phenotype. Although the process of differentiation is intimately linked to the cessation of proliferation, the mechanisms that dictate intestinal cell fate determination are not well characterized. To investigate the reprogramming of gene expression during the cell lineage allocation/differentiation process, we took advantage of a unique system of two clonal derivatives of HT29 cells, Cl16E and Cl19A cells, which spontaneously differentiate as mucus producing goblet and chloride-secreting cells, respectively, as a function of time. By profiling gene expression, we found that these two cell lines show remarkably similar kinetics of change in gene expression and common clusters of coordinately regulated genes. This demonstrates that lineage-specific differentiation of intestinal epithelial cells is characterized overall by the sequential recruitment of functionally similar gene sets independent of the final phenotype of the mature cells.
KW - Basal crypt secretory cells
KW - Gene expression
KW - Goblet cells
KW - Intestinal cell differentiation
KW - Microarray
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U2 - 10.1016/j.yexcr.2004.10.014
DO - 10.1016/j.yexcr.2004.10.014
M3 - Article
C2 - 15707571
AN - SCOPUS:13544251545
SN - 0014-4827
VL - 304
SP - 28
EP - 39
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -