Quantitative rather than qualitative differences in gene expression predominate in intestinal cell maturation along distinct cell lineages

Anna Velcich, Georgia Corner, Doru Paul, Min Zhuang, John M. Mariadason, Christian Laboisse, Leonard Augenlicht

Research output: Contribution to journalArticle

15 Scopus citations


Several cell types are present in the intestinal epithelium that likely arise from a common precursor, the stem cell, and each mature cell type expresses a unique set of genes that characterizes its functional phenotype. Although the process of differentiation is intimately linked to the cessation of proliferation, the mechanisms that dictate intestinal cell fate determination are not well characterized. To investigate the reprogramming of gene expression during the cell lineage allocation/differentiation process, we took advantage of a unique system of two clonal derivatives of HT29 cells, Cl16E and Cl19A cells, which spontaneously differentiate as mucus producing goblet and chloride-secreting cells, respectively, as a function of time. By profiling gene expression, we found that these two cell lines show remarkably similar kinetics of change in gene expression and common clusters of coordinately regulated genes. This demonstrates that lineage-specific differentiation of intestinal epithelial cells is characterized overall by the sequential recruitment of functionally similar gene sets independent of the final phenotype of the mature cells.

Original languageEnglish (US)
Pages (from-to)28-39
Number of pages12
JournalExperimental Cell Research
Issue number1
Publication statusPublished - Mar 10 2005



  • Basal crypt secretory cells
  • Gene expression
  • Goblet cells
  • Intestinal cell differentiation
  • Microarray

ASJC Scopus subject areas

  • Cell Biology

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