Quantitative proteomics reveals novel insights into isoniazid susceptibility in mycobacteria mediated by a universal stress protein

Xinling Hu, Xiaojing Li, Lige Huang, John Chan, Yuling Chen, Haiteng Deng, Kaixia Mi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Tuberculosis (TB) is caused by the ancient pathogen, Mycobacterium tuberculosis, and is one of the most serious infectious diseases in the world. Isoniazid (INH) is an important first-line drug for the treatment of active and latent TB. INH resistance is an increasing problem in the treatment of TB. Phenotypic resistance to INH, however, is poorly understood. In this study, we constructed a strain of Mycobacterium bovis BCG that overexpresses the latency-related universal stress protein (USP), BCG-2013, and designated this strain BCG-2013. BCG-2013 overexpression increased susceptibility to INH compared with that of the wild-type strain, BCG-pMV261. Quantitative proteomic analysis revealed that BCG-2013 overexpression resulted in the upregulation of 50 proteins and the downregulation of 26 proteins among the 1500 proteins identified. Upregulation of catalase-peroxidase KatG expression in BCG-2013 was observed and confirmed by qPCR, whereas expression of other INH resistance-related proteins did not change. In addition, differential expression of the mycobacterial persistence regulator MprA and its regulatory proteins was observed. BCG-2013 and katG mRNA levels increased in a Wayne dormancy model, whereas MprA mRNA levels decreased. Taken together, our results suggest that the increase in KatG levels induced by increased BCG-2013 levels underlies the phenotypic susceptibility of mycobacteria to INH.

Original languageEnglish (US)
Pages (from-to)1445-1454
Number of pages10
JournalJournal of Proteome Research
Volume14
Issue number3
DOIs
StatePublished - Mar 6 2015

Fingerprint

Isoniazid
Mycobacterium
Mycobacterium bovis
Heat-Shock Proteins
Proteomics
Proteins
Messenger RNA
Pathogens
Catalase
Peroxidase
Tuberculosis
Up-Regulation
Latent Tuberculosis
Mycobacterium tuberculosis
Pharmaceutical Preparations
Communicable Diseases
Down-Regulation

Keywords

  • isoniazid
  • mycobacteria
  • peroxidase-catalase KatG
  • quantitative proteomics
  • Universal stress protein BCG-2013

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Quantitative proteomics reveals novel insights into isoniazid susceptibility in mycobacteria mediated by a universal stress protein. / Hu, Xinling; Li, Xiaojing; Huang, Lige; Chan, John; Chen, Yuling; Deng, Haiteng; Mi, Kaixia.

In: Journal of Proteome Research, Vol. 14, No. 3, 06.03.2015, p. 1445-1454.

Research output: Contribution to journalArticle

Hu, Xinling ; Li, Xiaojing ; Huang, Lige ; Chan, John ; Chen, Yuling ; Deng, Haiteng ; Mi, Kaixia. / Quantitative proteomics reveals novel insights into isoniazid susceptibility in mycobacteria mediated by a universal stress protein. In: Journal of Proteome Research. 2015 ; Vol. 14, No. 3. pp. 1445-1454.
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