Quantitative Profiling of the Lymph Node Clearance Capacity

Cristina C. Clement, Wei Wang, Monika Dzieciatkowska, Marco Cortese, Kirk C. Hansen, Aniuska Becerra, Sangeetha Thangaswamy, Irina Nizamutdinova, Jee Young Moon, Lawrence J. Stern, Anatoliy A. Gashev, David Zawieja, Laura Santambrogio

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Transport of tissue-derived lymphatic fluid and clearance by draining lymph nodes are pivotal for maintenance of fluid homeostasis in the body and for immune-surveillance of the self- and non-self-proteomes. Yet a quantitative analysis of nodal filtration of the tissue-derived proteome present in lymphatic fluid has not been reported. Here we quantified the efficiency of nodal clearance of the composite proteomic load using label-free and isotope-labeling proteomic analysis of pre-nodal and post-nodal samples collected by direct cannulation. These results were extended by quantitation of the filtration efficiency of fluorophore-labeled proteins, bacteria, and beads infused at physiological flow rates into pre-nodal lymphatic collectors and collected by post-nodal cannulation. We developed a linear model of nodal filtration efficiency dependent on pre-nodal protein concentrations and molecular weight, and uncovered criteria for disposing the proteome incoming from defined anatomical districts under physiological conditions. These findings are pivotal to understanding the maximal antigenic load sustainable by a draining node, and promote understanding of pathogen spreading and nodal filtration of tumor metastasis, potentially helping to improve design of vaccination protocols, immunization strategies and drug delivery.

Original languageEnglish (US)
Article number11253
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Proteome
Lymph Nodes
Catheterization
Proteomics
Nodal Protein
Isotope Labeling
Lymphoid Tissue
Linear Models
Immunization
Vaccination
Homeostasis
Molecular Weight
Maintenance
Neoplasm Metastasis
Bacteria
Pharmaceutical Preparations
Neoplasms
Proteins

ASJC Scopus subject areas

  • General

Cite this

Clement, C. C., Wang, W., Dzieciatkowska, M., Cortese, M., Hansen, K. C., Becerra, A., ... Santambrogio, L. (2018). Quantitative Profiling of the Lymph Node Clearance Capacity. Scientific Reports, 8(1), [11253]. https://doi.org/10.1038/s41598-018-29614-0

Quantitative Profiling of the Lymph Node Clearance Capacity. / Clement, Cristina C.; Wang, Wei; Dzieciatkowska, Monika; Cortese, Marco; Hansen, Kirk C.; Becerra, Aniuska; Thangaswamy, Sangeetha; Nizamutdinova, Irina; Moon, Jee Young; Stern, Lawrence J.; Gashev, Anatoliy A.; Zawieja, David; Santambrogio, Laura.

In: Scientific Reports, Vol. 8, No. 1, 11253, 01.12.2018.

Research output: Contribution to journalArticle

Clement, CC, Wang, W, Dzieciatkowska, M, Cortese, M, Hansen, KC, Becerra, A, Thangaswamy, S, Nizamutdinova, I, Moon, JY, Stern, LJ, Gashev, AA, Zawieja, D & Santambrogio, L 2018, 'Quantitative Profiling of the Lymph Node Clearance Capacity', Scientific Reports, vol. 8, no. 1, 11253. https://doi.org/10.1038/s41598-018-29614-0
Clement CC, Wang W, Dzieciatkowska M, Cortese M, Hansen KC, Becerra A et al. Quantitative Profiling of the Lymph Node Clearance Capacity. Scientific Reports. 2018 Dec 1;8(1). 11253. https://doi.org/10.1038/s41598-018-29614-0
Clement, Cristina C. ; Wang, Wei ; Dzieciatkowska, Monika ; Cortese, Marco ; Hansen, Kirk C. ; Becerra, Aniuska ; Thangaswamy, Sangeetha ; Nizamutdinova, Irina ; Moon, Jee Young ; Stern, Lawrence J. ; Gashev, Anatoliy A. ; Zawieja, David ; Santambrogio, Laura. / Quantitative Profiling of the Lymph Node Clearance Capacity. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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