Quantitative peptidomics to measure neuropeptide levels in animal models relevant to psychiatric disorders

Julia S. Gelman, Jonathan Wardman, Vadiraja B. Bhat, Fabio C. Gozzo, Lloyd D. Fricker

Research output: Chapter in Book/Report/Conference proceedingChapter

16 Citations (Scopus)

Abstract

Neuropeptides play many important roles in cell-cell signaling and are involved in the control of anxiety, depression, pain, reward pathways, and many other processes that are relevant to psychiatric disorders. Mass spectrometry-based peptidomics techniques can identify the precise forms of peptides that are present in a given tissue. Utilizing this technique, peptides with any posttranslational modifications can be identified, and the exact sequence of the peptides can be determined. Unlike radioimmunoassays, which are limited by specific antibodies and often cannot discriminate between different lengths of peptides from the same precursor, peptidomics reveals the precise sequence and allows for the identification of both known and novel peptides. The use of isotopic labels allows for quantitative peptidomics, which results in the ability to compare peptide levels between differently treated samples. These tags can be synthesized in five different isotopic forms, permitting multivariate analysis of up to five different groups of tissue extracts in a single liquid chromatography/mass spectrometry run; this is ideal for measuring changes in neuropeptides in animals subjected to drug treatments, or in comparing animal models of psychiatric disorders.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages487-503
Number of pages17
Volume829
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume829
ISSN (Print)10643745

Fingerprint

Neuropeptides
Psychiatry
Animal Models
Peptides
Mass Spectrometry
Aptitude
Tissue Extracts
Post Translational Protein Processing
Reward
Liquid Chromatography
Radioimmunoassay
Multivariate Analysis
Anxiety
Depression
Pain
Antibodies
Pharmaceutical Preparations

Keywords

  • Neuropeptides
  • Peptidase
  • Peptidomics
  • Protease
  • Proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Gelman, J. S., Wardman, J., Bhat, V. B., Gozzo, F. C., & Fricker, L. D. (2012). Quantitative peptidomics to measure neuropeptide levels in animal models relevant to psychiatric disorders. In Methods in Molecular Biology (Vol. 829, pp. 487-503). (Methods in Molecular Biology; Vol. 829). https://doi.org/10.1007/978-1-61779-458-2_31

Quantitative peptidomics to measure neuropeptide levels in animal models relevant to psychiatric disorders. / Gelman, Julia S.; Wardman, Jonathan; Bhat, Vadiraja B.; Gozzo, Fabio C.; Fricker, Lloyd D.

Methods in Molecular Biology. Vol. 829 2012. p. 487-503 (Methods in Molecular Biology; Vol. 829).

Research output: Chapter in Book/Report/Conference proceedingChapter

Gelman, JS, Wardman, J, Bhat, VB, Gozzo, FC & Fricker, LD 2012, Quantitative peptidomics to measure neuropeptide levels in animal models relevant to psychiatric disorders. in Methods in Molecular Biology. vol. 829, Methods in Molecular Biology, vol. 829, pp. 487-503. https://doi.org/10.1007/978-1-61779-458-2_31
Gelman JS, Wardman J, Bhat VB, Gozzo FC, Fricker LD. Quantitative peptidomics to measure neuropeptide levels in animal models relevant to psychiatric disorders. In Methods in Molecular Biology. Vol. 829. 2012. p. 487-503. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-458-2_31
Gelman, Julia S. ; Wardman, Jonathan ; Bhat, Vadiraja B. ; Gozzo, Fabio C. ; Fricker, Lloyd D. / Quantitative peptidomics to measure neuropeptide levels in animal models relevant to psychiatric disorders. Methods in Molecular Biology. Vol. 829 2012. pp. 487-503 (Methods in Molecular Biology).
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